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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • SOX9 Antibodies

          Invitrogen

          SOX9 Polyclonal Antibody

          Advanced Verification
          6 Published Figures
          9 References
          View all (64) SOX9 antibodies

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          Cite SOX9 Polyclonal Antibody

          • Antibody Testing Data (7)
          • Published Figures (6)
          • Advanced Verification (2)
          SOX9 Antibody in Immunocytochemistry (ICC/IF)
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          SOX9 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 15

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          SOX9 Antibody (PA5-81966) in ICC/IF

          Immunofluorecent analysis of SOX9 in human cell line U-251 MG using SOX9 Polyclonal Antibody (Product # PA5-81966). Staining shows localization to nucleoplasm. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          SOX9 Antibody in Immunocytochemistry (ICC/IF)
          SOX9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SOX9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SOX9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SOX9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SOX9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          SOX9 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody
          SOX9 Antibody
          SOX9 Polyclonal Antibody

          Product Details

          PA5-81966

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.04-0.4 µg/mL
          View 5 publications 5 publications

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:500-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          0.25-2 µg/mL
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          C. elegans, Human, Mouse, Rat, Xenopus

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein corresponding to Human SOX9. Recombinant protein control fragment (Product #RP-100725).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.10 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 40% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2789127

          Product Specific Information

          Immunogen sequence: SQRTHIKTEQ LSPSHYSEQQ QHSPQQIAYS PFNLPHYSPS YPPITRSQYD YTDHQNSSSY YSHAAGQGTG LYSTFTYMNP AQRPMYTPIA DTSGVPSIPQ THSPQHWEQP VYTQLTR

          Highest antigen sequence indentity to the following orthologs: Rat - 96%, Mouse - 97%.

          Target Information

          SOX9 has a role in sex determination and differentiation of Sertoli cells. It is involved in chondrogenesis and regulates the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Translocation of this gene can cause campomelic dysplasia. SOX9 is involved in the formation of testes from the indifferent fetal gonads. It is a major molecular component of the neuron-glia switch in developing spinal cord. During normal development, SOX9 allows the prostate epithelium to outgrow into the mesenchyme and then provides basal cell support for development and maintenance of the luminal epithelium. These functions of SOX9 are subverted in prostate cancer to support tumor growth and invasion. SOX9 may direct the formation of neural crest precursors and the development of a range of neural crest derivative. It has a transcriptional regulation in melanin production in cells. Northern blot analysis shows its expression on adult testis, adult heart, and fetal brain.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: mSox9; SOX 9; SRY (sex determining region Y)-box 9; SRY (sex determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal); SRY (sex determining region Y)-box9; SRY (sex-determining region Y)-box 9 protein; SRY box 9; SRY-Box 9 ; SRY-box containing gene 9; SRY-related HMG-box, gene 9; Transcription factor SOX-9

          View more View less

          Gene Aliases: 2010306G03Rik; AV220920; CMD1; CMPD1; mKIAA4243; Sox-9; SOX9; SRA1; SRXX2; SRXY10

          View more View less

          UniProt ID: (Human) P48436, (Rat) F1LYL9, (Mouse) Q04887

          View more View less

          Entrez Gene ID: (Human) 6662, (Rat) 140586, (Mouse) 20682

          View more View less

          Function(s)
          core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding enhancer sequence-specific DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein kinase activity protein binding beta-catenin binding protein kinase A catalytic subunit binding enhancer binding bHLH transcription factor binding protein heterodimerization activity pre-mRNA intronic binding transcription regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding sequence-specific DNA binding transcription regulatory region DNA binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter skeletal system development cartilage condensation ossification branching involved in ureteric bud morphogenesis cell fate specification epithelial to mesenchymal transition tissue homeostasis positive regulation of protein phosphorylation hair follicle development positive regulation of mesenchymal cell proliferation chondrocyte development negative regulation of immune system process heart valve development heart valve morphogenesis heart valve formation endocardial cushion morphogenesis chondrocyte differentiation involved in endochondral bone morphogenesis chondrocyte hypertrophy nucleosome assembly chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein complex assembly protein phosphorylation cytoskeleton organization signal transduction epidermal growth factor receptor signaling pathway spermatogenesis heart development positive regulation of cell proliferation negative regulation of cell proliferation male gonad development regulation of cell cycle process positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration neural crest cell development positive regulation of phosphatidylinositol 3-kinase signaling single organismal cell-cell adhesion male germ-line sex determination cAMP-mediated signaling cell differentiation regulation of cell adhesion extracellular matrix organization male sex determination negative regulation of ossification negative regulation of bone mineralization prostate gland development negative regulation of epithelial cell differentiation positive regulation of epithelial cell differentiation mammary gland development notochord development otic vesicle formation endocrine pancreas development negative regulation of chondrocyte differentiation positive regulation of chondrocyte differentiation lacrimal gland development protein localization to nucleus somatic stem cell population maintenance intrahepatic bile duct development regulation of cell proliferation regulation of apoptotic process negative regulation of apoptotic process protein kinase B signaling cell fate commitment regulation of cell differentiation negative regulation of myoblast differentiation positive regulation of protein catabolic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of photoreceptor cell differentiation oligodendrocyte differentiation homeostasis of number of cells within a tissue positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation cartilage development Sertoli cell differentiation Sertoli cell development astrocyte fate commitment retina development in camera-type eye limb bud formation retinal rod cell differentiation endochondral bone morphogenesis epithelial tube branching involved in lung morphogenesis lung epithelial cell differentiation prostate gland morphogenesis epithelial cell proliferation involved in prostatic bud elongation bronchus cartilage development trachea cartilage development intestinal epithelial structure maintenance regulation of cell proliferation involved in tissue homeostasis positive regulation of cartilage development regulation of branching involved in lung morphogenesis morphogenesis of a branching epithelium lung smooth muscle development negative regulation of biomineral tissue development ERK1 and ERK2 cascade Harderian gland development cellular response to mechanical stimulus cellular response to retinoic acid cellular response to interleukin-1 cellular response to epidermal growth factor stimulus cellular response to heparin cellular response to transforming growth factor beta stimulus renal vesicle induction ureter development ureter urothelium development ureter smooth muscle cell differentiation ureter morphogenesis metanephric nephron tubule formation negative regulation of canonical Wnt signaling pathway cochlea morphogenesis positive regulation of kidney development positive regulation of branching involved in ureteric bud morphogenesis positive regulation of extracellular matrix assembly positive regulation of male gonad development positive regulation of cell proliferation involved in heart morphogenesis positive regulation of mesenchymal stem cell differentiation regulation of epithelial cell proliferation involved in lung morphogenesis negative regulation of mesenchymal cell apoptotic process Notch signaling pathway
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