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Invitrogen
SP1 Polyclonal Antibody
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FIGURE: 1 / 1
SP1 Antibody (PA1-30332) in WB
Aging and disease 2023 -
Figure 7. Role of Sp1 in Nef-induced GFAP downmodulation. U373 were transfected with C3 or Nef and harvested for Western blotting against an anti-Sp1, Nef, or GAPDH antibody (A), followed by Sp1 quantitation using the loading control GAPDH as the reference (B); or U373 were transfected with pDeltaSp1-GFAP.luc plasmid, cultured for 16 hr, split into two equal samples, and infected with VSVG/GAGi and GAGi.Nef... View More
Product Details
PA1-30332
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide mapping to an internal domain of human Sp1.
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 0.2% gelatin
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-30332 detects SP1 from human, rat, mouse samples.
Target Information
SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HGNC:11205; Specificity protein 1; trans-acting transcription factor 1; Transcription factor Sp1
Gene Aliases: 1110003E12Rik; AA450830; AI845540; SP1; Sp1-1; TSFP1
UniProt ID: (Human) P08047, (Mouse) O89090, (Rat) Q01714
Entrez Gene ID: (Human) 6667, (Mouse) 20683, (Rat) 24790
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
core promoter sequence-specific DNA binding
RNA polymerase II repressing transcription factor binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein C-terminus binding
transcription factor binding
histone acetyltransferase binding
protein homodimerization activity
histone deacetylase binding
bHLH transcription factor binding
sequence-specific DNA binding
transcription regulatory region DNA binding
metal ion binding
HMG box domain binding
RNA polymerase II core promoter sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
nucleic acid binding
enhancer binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
C2H2 zinc finger transcription factor
regulation of transcription, DNA-templated
viral process
snRNA transcription from RNA polymerase II promoter
positive regulation by host of viral transcription
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
regulation of phenotypic switching by transcription from RNA polymerase II promoter
positive regulation of hydrogen sulfide biosynthetic process
ossification
in utero embryonic development
trophectodermal cell differentiation
liver development
embryonic placenta development
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
megakaryocyte differentiation
lung development
enucleate erythrocyte differentiation
embryonic camera-type eye morphogenesis
embryonic skeletal system development
embryonic process involved in female pregnancy
definitive hemopoiesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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