Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
SP1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. SP1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences.
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Protein Aliases: specificity protein 1; Transcription factor Sp1
Gene Aliases: SP1; TSFP1
UniProt ID: (Human) Q9NYE7
Entrez Gene ID: (Human) 6667