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Invitrogen

SP1 Polyclonal Antibody

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View all (39) SP1 antibodies

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SP1 Polyclonal Antibody

Product Details

PA5-95583

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-

ELISA (ELISA)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human SP1 recombinant protein (Position: Q384-A603).
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2807385

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Jurkat whole cell, human A431 whole cell, human 293T whole cell, human Hela whole cell. IHC: human non-samll cell lung carcinoma tissue, human ovarian cancer tissue, mouse lung tissue, rat lung tissue. ICC/IF: A549 cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: HGNC:11205; Specificity protein 1; trans-acting transcription factor 1; transcription factor; transcription factor Sp-1; Transcription factor Sp1; transcriptional activator protein; unnamed protein product

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Gene Aliases: 1110003E12Rik; AA450830; AI845540; SP1; Sp1-1; TSFP1

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UniProt ID: (Human) P08047, (Mouse) O89090, (Rat) Q01714

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Entrez Gene ID: (Human) 6667, (Mouse) 20683, (Rat) 24790

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription cofactor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding histone acetyltransferase binding identical protein binding protein homodimerization activity histone deacetylase binding bHLH transcription factor binding sequence-specific DNA binding metal ion binding binding, bridging RNA polymerase II sequence-specific DNA binding transcription factor binding sequence-specific double-stranded DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II repressing transcription factor binding nucleic acid binding protein C-terminus binding transcription factor binding enhancer binding transcription regulatory region DNA binding HMG box domain binding C2H2 zinc finger transcription factor
Process(es)
regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter positive regulation of gene expression cellular response to insulin stimulus response to hydroperoxide cellular response to zinc ion starvation positive regulation of blood vessel endothelial cell migration positive regulation by host of viral transcription positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process cellular response to estrogen stimulus positive regulation of beta-amyloid formation cellular response to wortmannin positive regulation of hydrogen sulfide biosynthetic process positive regulation of vascular endothelial cell proliferation positive regulation of apoptotic signaling pathway ossification in utero embryonic development trophectodermal cell differentiation liver development embryonic placenta development transcription, DNA-templated transcription from RNA polymerase II promoter megakaryocyte differentiation lung development enucleate erythrocyte differentiation embryonic camera-type eye morphogenesis embryonic skeletal system development embryonic process involved in female pregnancy definitive hemopoiesis
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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