Immunohistochemical staining of SP100 in human colon, lymph node, spleen and testis using SP100 Polyclonal Antibody (Product # PA5-53476) (A) shows similar protein distribution across tissues to an independent SP100 Polyclonal Antibody (B).

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SP100 Antibody — Relative expression in western blot: Antibody specificity was demonstrated by detection of known differential basal expression of the target across tissue/cell models. Expression of SP100 was observed specifically in SK-MEL-30 cells and in HEK293 cells using anti-SP100 Polyclonal Antibody (Product # PA5-53476) in western blot. The relative expression levels of SP100 within each cell line is shown using RNA-Seq. {RE}

Immunogen image

Immunogen Image SP100 Polyclonal Antibody

Product Details

PA5-53476

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

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Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human SP100. Recombinant protein control fragment (Product #RP-91589).

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2647754

Product Specific Information

Immunogen sequence: QARTHHNQAS DIIVISSEDS EGSTDVDEPL EVFISAPRSE PVINNDNPLE SNDEKEGQEA TCSRPQIVPE PMDFRKLSTF RESFKKRVIG QDHDFSESSE

Highest antigen sequence identity to the following orthologs: Mouse - 24%, Rat - 26%.

Target Information

This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp686E07254; FLJ00340; FLJ34579; nuclear autoantigen; Nuclear autoantigen Sp-100; Nuclear dot-associated Sp100 protein; SP 100; SP100-HMG nuclear autoantigen; Speckled 100 kDa; splice variant; unnamed protein product

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Gene Aliases: lysp100b; SP100

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UniProt ID: (Human) P23497

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Entrez Gene ID: (Human) 6672

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription corepressor activity protein binding kinase binding protein domain specific binding identical protein binding protein homodimerization activity protein dimerization activity RNA polymerase II sequence-specific DNA binding transcription factor binding chromo shadow domain binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter telomere maintenance DNA metabolic process regulation of transcription from RNA polymerase II promoter defense response immune response negative regulation of endothelial cell migration DNA damage response, signal transduction by p53 class mediator response to retinoic acid negative regulation of viral transcription response to cytokine response to type I interferon response to interferon-gamma maintenance of protein location regulation of angiogenesis negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of protein export from nucleus retinoic acid receptor signaling pathway regulation of viral process interferon-gamma-mediated signaling pathway type I interferon signaling pathway regulation of extrinsic apoptotic signaling pathway via death domain receptors regulation of Fas signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SP100 Polyclonal Antibody

SP100 Antibody (PA5-53476) in IHC

Product Details

PA5-53476

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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