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Invitrogen
SP100 Polyclonal Antibody
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FIGURE: 1 / 2
SP100 Antibody (PA5-78177) in ICC/IF
SP100 Polyclonal Antibody detects SP100 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SP100 protein stained by SP100 Polyclonal Antibody (Product # PA5-78177) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Product Details
PA5-78177
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human SP100. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.68 mg/mL
Amount
68 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: Jurkat, Raji, NCI-H929
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686E07254; FLJ00340; FLJ34579; nuclear autoantigen; Nuclear autoantigen Sp-100; Nuclear dot-associated Sp100 protein; SP 100; SP100-HMG nuclear autoantigen; Speckled 100 kDa; splice variant; unnamed protein product
Gene Aliases: lysp100b; SP100
UniProt ID: (Human) P23497
Entrez Gene ID: (Human) 6672
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription corepressor activity
protein binding
kinase binding
protein domain specific binding
identical protein binding
protein homodimerization activity
protein dimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
chromo shadow domain binding
negative regulation of transcription from RNA polymerase II promoter
telomere maintenance
DNA metabolic process
regulation of transcription from RNA polymerase II promoter
defense response
immune response
negative regulation of endothelial cell migration
DNA damage response, signal transduction by p53 class mediator
response to retinoic acid
negative regulation of viral transcription
response to cytokine
response to type I interferon
response to interferon-gamma
maintenance of protein location
regulation of angiogenesis
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of protein export from nucleus
retinoic acid receptor signaling pathway
regulation of viral process
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
regulation of extrinsic apoptotic signaling pathway via death domain receptors
regulation of Fas signaling pathway
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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