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Invitrogen
SP3 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 10
SP3 Antibody (PA5-78176) in ICC/IF
SP3 Polyclonal Antibody detects SP3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SP3 stained by SP3 Polyclonal Antibody (Product # PA5-78176) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.
Product Details
PA5-78176
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human SP3. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2736652
Product Specific Information
Positive Control: HeLa, Jurkat, Jurkat_NE, Raji, NCI-H929^M
^M
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp686O1631; GC-binding transcription factor Sp3; specificity protein 3; SPR-2; SPR1; Transcription factor Sp3
Gene Aliases: SP3; SPR2
UniProt ID: (Human) Q02447
Entrez Gene ID: (Human) 6670
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
chromatin binding
protein binding
metal ion binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
ossification
natural killer cell differentiation
trophectodermal cell differentiation
liver development
embryonic placenta development
transcription, DNA-templated
regulation of transcription, DNA-templated
protein sumoylation
B cell differentiation
T cell differentiation
megakaryocyte differentiation
monocyte differentiation
lung development
granulocyte differentiation
enucleate erythrocyte differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryonic camera-type eye morphogenesis
embryonic skeletal system development
embryonic process involved in female pregnancy
definitive hemopoiesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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