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          • Primary Antibodies ›
          • SP4 Antibodies
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          Invitrogen

          SP4 Polyclonal Antibody

          View all (6) SP4 antibodies

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          Cite SP4 Polyclonal Antibody

          • Antibody Testing Data (8)
          SP4 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          SP4 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          SP4 Antibody (PA5-118276) in ICC/IF

          Immunofluorescence staining of SP4 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with SP4 Polyclonal Antibody (Product # PA5-118276, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SP4 Antibody in Immunocytochemistry (ICC/IF)
          SP4 Antibody in Immunocytochemistry (ICC/IF)
          SP4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SP4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SP4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SP4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SP4 Antibody in Western Blot (WB)
          SP4 Antibody in Western Blot (WB)

          Product Details

          PA5-118276

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          -
          Product Specifications

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E. coli-derived Human SP4 fragment

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Wet ice

          RRID

          AB_2902873

          Target Information

          This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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