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Invitrogen
SP4 Polyclonal Antibody
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FIGURE: 1 / 1
SP4 Antibody (PA5-80058) in WB
Western blot analysis of SP4 using SP4 Polyclonal Antibody (Product # PA5-80058). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. Lane 2: human K562 whole cell lysates. Lane 3: rat brain tissue lysates. Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked... View More
Product Details
PA5-80058
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SP4 (29-44aa ENNNKKPKTSGSQDSQ).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Jurkat whole cell, human K562 whole cell, rat brain tissue, mouse brain tissue.
Target Information
This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HF-1b zinc finger protein; MGC130008; MGC130009; SPR-1; trans-acting transcription factor 4; Transcription factor Sp4; unnamed protein product
Gene Aliases: 5730497N03Rik; HF-1b; HF1-b; HF1B; SP4; SPR-1
UniProt ID: (Human) Q02446
Entrez Gene ID: (Human) 6671, (Rat) 25162, (Mouse) 20688
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
identical protein binding
sequence-specific DNA binding
metal ion binding
transcription coactivator activity
nucleic acid binding
C2H2 zinc finger transcription factor
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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