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SP4 Antibody (PA5-80059) in WB
Western blot analysis of SP4 in Lane 1: rat brain tissue lysate, Lane 2: mouse brain tissue lysate, Lane 3: U87 whole cell lysate, Lane 4: HeLa whole cell lysate using 40-50 µg per well. Sample was incubated with SP4 (Product # PA5-80059) at a dilution of 0.5 µg/mL.
Product Details
PA5-80059
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SP4 (592-620aa AVSPDQLTQVHLQQGQQTSDQEVQPGKRL).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2747174
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HF-1b zinc finger protein; MGC130008; MGC130009; SPR-1; trans-acting transcription factor 4; Transcription factor Sp4
Gene Aliases: 5730497N03Rik; HF-1b; HF1-b; HF1B; SP4; SPR-1
UniProt ID: (Human) Q02446
Entrez Gene ID: (Human) 6671, (Rat) 25162, (Mouse) 20688
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