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FIGURE: 1 / 1
SP4 Antibody (PA5-95764) in WB
Western blot analysis of SP4 in extracts of various cell lines. Samples were incubated with SP4 Polyclonal antibody (Product # PA5-95764) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-95764
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 280-440 of human SP4 (NP_0031032)
Conjugate
Form
Liquid
Concentration
2.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2807566
Product Specific Information
Immunogen sequence: TGQVGQPAAT ADSGTSNGNQ LVSTPTNTTT SASTMPESPS SSTTCTTTAS TSLTSSDTLV SSADTGQYAS TSASSSERTI EESQTPAATE SEAQSSSQLQ PNGMQNAQDQ SNSLQQVQIV GQPILQQIQI QQPQQQIIQA IPPQSFQLQS GQTIQTIQQQ P; Positive Samples: Mouse thymus, Mouse spleen, Mouse pancreas, Rat thymus; Cellular Location: Nucleus
Target Information
This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HF-1b zinc finger protein; MGC130008; MGC130009; SPR-1; trans-acting transcription factor 4; Transcription factor Sp4
Gene Aliases: 5730497N03Rik; HF-1b; HF1-b; HF1B; SP4; SPR-1
UniProt ID: (Human) Q02446
Entrez Gene ID: (Human) 6671, (Rat) 25162, (Mouse) 20688
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