Invitrogen

SP7 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (9) SP7 antibodies

Datasheet

Protocols

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Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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SP7 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of human intestine tissue using an anti-SP7 polyclonal antibody (Product # PA5-40411).

SP7 Antibody in Western Blot (WB) — Western blot analysis of human Jurkat whole cell lysate using an anti-SP7 polyclonal antibody (Product # PA5-40411).

SP7 Antibody — Fig 7 (A) Immunofluorescence staining of Osterix (red) and nuclei (blue). Calvarial cells were treated with conditioned media or 5mM calcium alone, or in combination for 48 hours. Scale bar 20X. (B) Western blot analyses of cell lysates was performed to detect the Osterix and Runx2 protein levels after the treatment with the different conditions for 48 hours. GAPDH was used as internal control. {TM}

Product Details

PA5-40411

Applications	Tested Dilution	Publications
Western Blot (WB)	0.2-1 µg/mL	View 1 publication 1 publication
Immunohistochemistry (IHC)	5 µg/mL	-

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide directed towards the C-terminal of human SP7 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS with 2% sucrose
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2606426

Product Specific Information

Peptide sequence: RTHGEPGPGP PPSGPKELGE GRSTGEEEAS QTPRPSASPA TPEKAPGGSP

Sequence homology: Cow: 100%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 92%; Rabbit: 86%; Rat: 93%

Target Information

SP7 is a C2H2-type zinc finger transcription factor of the SP gene family and a putative master regulator of bone cell differentiation (Gao et al., 2004 [PubMed 15474293]).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.