Proteintech

SPAG5 Polyclonal Antibody

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Antibody Testing Data (16)

FIGURE: 1 / 16

SPAG5 Antibody (14726-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 14726-1-AP) SPAG5 antibody at dilution of 1:1,000 incubated at room temperature for 1.5 hours.

Product Image Gallery

SPAG5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPAG5 antibody (Product # 14726-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SPAG5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 14726-1-AP (SPAG5 antibody) at dilution of 1:100 (under 40x lens).

SPAG5 Antibody in Immunoprecipitation (IP) — IP result of anti-SPAG5 (IP:14726-1-AP, 5ug; Detection:14726-1-AP 1:1000) with HeLa cells lysate 3000ug.

Product Details

14726-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 7 publications 7 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SPAG5 Fusion Protein Ag6485 (844-1193 aa encoded by BC000322)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.36 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody is specific to SPAG5.

Immunogen sequence: TVENLTAKL ASTIADNQEQ DLEKTRQYSQ KLGLLTEQLQ SLTLFLQTKL KEKTEQETLL LSTACPPTQE HPLPNDRTFL GSILTAVADE EPESTPVPLL GSDKSAFTRV ASMVSLQPAE TPGMEESLAE MSIMTTELQS LCSLLQESKE EAIRTLQRKI CELQARLQAQ EEQHQEVQKA KEADIEKLNQ ALCLRYKNEK ELQEVIQQQN EKILEQIDKS GELISLREEV THLTRSLRRA ETETKVLQEA LAGQLDSNCQ PMATNWIQEK VWLSQEVDKL RVMFLEMKNE KEKLMIKFQS HRNILEENLR RSDKELEKLD DIVQHIYKTL LSIPEVVRGC KELQGLLEFL S (844-1193 aa encoded by BC000322)

Target Information

SPAG5 (sperm associated antigen 5), also known as MAP126, hMAP126, DEEPEST or Astrin, is a 1,193 amino acid protein that is expressed at high levels in testis and at lower levels in liver, placenta, pancreas, colon and thymus. Localized specifically to spindle poles during prophase and throughout the spindle during metaphase and anaphase, SPAG5 is essential for proper spindle formation and is thought to be involved in the dynamic and functional regulation of mitotic spindles. Additionally, SPAG5 plays a role in sister chromatid cohesion and subsequent chromatid separation and, via its association with chromatids, is an important regulator of chromosome integrity. SPAG5 has a long stalk and a globular head domain through which if forms a homodimer that is characterized by aster-like structures. Upon DNA damage, SPAG5 may be phosphorylated by ATR or ATM.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Upregulation of ENKD1 disrupts cellular homeostasis to promote lymphoma development.

Journal of cellular physiology

Song T,He N,Hao Z,Yang Y

Thu Jun 01 00:00:00 EDT 2023

The splicing factor SF3B4 drives proliferation and invasion in cervical cancer by regulating SPAG5.

Cell death discovery

Li Y,Diao Y,Wang Z,Wang S,Peng J,Kong B

Tue Jul 19 00:00:00 EDT 2022

CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72.

BMC biology

Ying Z,Wang K,Wu J,Wang M,Yang J,Wang X,Zhou G,Chen H,Xu H,Sze SCW,Gao F,Li C,Sha O

Mon Oct 24 00:00:00 EDT 2022

CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72.

BMC biology

Ying Z,Wang K,Wu J,Wang M,Yang J,Wang X,Zhou G,Chen H,Xu H,Sze SCW,Gao F,Li C,Sha O

Mon Oct 24 00:00:00 EDT 2022

Downregulation of sperm-associated antigen 5 inhibits melanoma progression by regulating forkhead box protein M1/A disintegrin and metalloproteinase 17/NOTCH1 signaling.

Bioengineered

Dang L,Shi C,Zhang Q,Liao P,Wang Y

Tue Mar 01 00:00:00 EST 2022

Downregulation of sperm-associated antigen 5 inhibits melanoma progression by regulating forkhead box protein M1/A disintegrin and metalloproteinase 17/NOTCH1 signaling.

Bioengineered

Dang L,Shi C,Zhang Q,Liao P,Wang Y

Tue Mar 01 00:00:00 EST 2022

CLUH controls astrin-1 expression to couple mitochondrial metabolism to cell cycle progression.

eLife

Schatton D,Di Pietro G,Szczepanowska K,Veronese M,Marx MC,Braunöhler K,Barth E,Müller S,Giavalisco P,Langer T,Trifunovic A,Rugarli EI

Fri May 13 00:00:00 EDT 2022

CLUH controls astrin-1 expression to couple mitochondrial metabolism to cell cycle progression.

eLife

Schatton D,Di Pietro G,Szczepanowska K,Veronese M,Marx MC,Braunöhler K,Barth E,Müller S,Giavalisco P,Langer T,Trifunovic A,Rugarli EI

Fri May 13 00:00:00 EDT 2022

DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction.

eLife

Lau EO,Damiani D,Chehade G,Ruiz-Reig N,Saade R,Jossin Y,Aittaleb M,Schakman O,Tajeddine N,Gailly P,Tissir F

Mon Apr 26 00:00:00 EDT 2021

DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction.

eLife

Lau EO,Damiani D,Chehade G,Ruiz-Reig N,Saade R,Jossin Y,Aittaleb M,Schakman O,Tajeddine N,Gailly P,Tissir F

Mon Apr 26 00:00:00 EDT 2021

Fe-doped chrysotile nanotubes containing siRNAs to silence SPAG5 to treat bladder cancer.

Journal of nanobiotechnology

Liu J,Zhang Y,Zeng H,Wang L,Zhang Q,Wu P,Liu X,Xie H,Xiang W,Liu B,Liu J,Liu X,Xie J,Tang J,Long Z,He L,Xiao M,Xiang L,Cao K

Wed Jun 23 00:00:00 EDT 2021

Fe-doped chrysotile nanotubes containing siRNAs to silence SPAG5 to treat bladder cancer.

Journal of nanobiotechnology

Liu J,Zhang Y,Zeng H,Wang L,Zhang Q,Wu P,Liu X,Xie H,Xiang W,Liu B,Liu J,Liu X,Xie J,Tang J,Long Z,He L,Xiao M,Xiang L,Cao K

Wed Jun 23 00:00:00 EDT 2021

SPAG5-AS1 inhibited autophagy and aggravated apoptosis of podocytes via SPAG5/AKT/mTOR pathway.

Cell proliferation

Xu J,Deng Y,Wang Y,Sun X,Chen S,Fu G

Sat Feb 01 00:00:00 EST 2020

Kinetochores attached to microtubule-ends are stabilised by Astrin bound PP1 to ensure proper chromosome segregation.

eLife

Conti D,Gul P,Islam A,Martín-Durán JM,Pickersgill RW,Draviam VM

Fri Dec 06 00:00:00 EST 2019

p53 suppression is essential for oncogenic SPAG5 upregulation in lung adenocarcinoma.

Biochemical and biophysical research communications

Wang T,Li K,Song H,Xu D,Liao Y,Jing B,Guo W,Hu M,Kuang Y,Sun B,Ling J,Zhang T,Xu J,Yao F,Deng J

Tue May 28 00:00:00 EDT 2019

CDC20B is required for deuterosome-mediated centriole production in multiciliated cells.

Nature communications

Revinski DR,Zaragosi LE,Boutin C,Ruiz-Garcia S,Deprez M,Thomé V,Rosnet O,Gay AS,Mercey O,Paquet A,Pons N,Ponzio G,Marcet B,Kodjabachian L,Barbry P

Wed Nov 07 00:00:00 EST 2018

CDC20B is required for deuterosome-mediated centriole production in multiciliated cells.

Nature communications

Revinski DR,Zaragosi LE,Boutin C,Ruiz-Garcia S,Deprez M,Thomé V,Rosnet O,Gay AS,Mercey O,Paquet A,Pons N,Ponzio G,Marcet B,Kodjabachian L,Barbry P

Wed Nov 07 00:00:00 EST 2018

CDC20B is required for deuterosome-mediated centriole production in multiciliated cells.

Nature communications

Revinski DR,Zaragosi LE,Boutin C,Ruiz-Garcia S,Deprez M,Thomé V,Rosnet O,Gay AS,Mercey O,Paquet A,Pons N,Ponzio G,Marcet B,Kodjabachian L,Barbry P

Wed Nov 07 00:00:00 EST 2018

CDC20B is required for deuterosome-mediated centriole production in multiciliated cells.

Nature communications

Revinski DR,Zaragosi LE,Boutin C,Ruiz-Garcia S,Deprez M,Thomé V,Rosnet O,Gay AS,Mercey O,Paquet A,Pons N,Ponzio G,Marcet B,Kodjabachian L,Barbry P

Wed Nov 07 00:00:00 EST 2018

Phosphorylation of Astrin Regulates Its Kinetochore Function.

The Journal of biological chemistry

Chung HJ,Park JE,Lee NS,Kim H,Jang CY

Fri Aug 19 00:00:00 EDT 2016

Phosphorylation of Astrin Regulates Its Kinetochore Function.

The Journal of biological chemistry

Chung HJ,Park JE,Lee NS,Kim H,Jang CY

Fri Aug 19 00:00:00 EDT 2016

Phosphorylation of Astrin Regulates Its Kinetochore Function.

The Journal of biological chemistry

Chung HJ,Park JE,Lee NS,Kim H,Jang CY

Fri Aug 19 00:00:00 EDT 2016

Phosphorylation of Astrin Regulates Its Kinetochore Function.

The Journal of biological chemistry

Chung HJ,Park JE,Lee NS,Kim H,Jang CY

Fri Aug 19 00:00:00 EDT 2016

Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.

eLife