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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:1,000-1:4,000 | View 4 publications 4 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:500 | - |
Immunocytochemistry (ICC/IF) |
1:20-1:200 | View 3 publications 3 publications |
Immunoprecipitation (IP) |
0.5-4.0 µg | View 1 publication 1 publication |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Published species |
Human, Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
SPAG5 Fusion Protein Ag6485 (844-1193 aa encoded by BC000322) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.36 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
This antibody is specific to SPAG5.
Immunogen sequence: TVENLTAKL ASTIADNQEQ DLEKTRQYSQ KLGLLTEQLQ SLTLFLQTKL KEKTEQETLL LSTACPPTQE HPLPNDRTFL GSILTAVADE EPESTPVPLL GSDKSAFTRV ASMVSLQPAE TPGMEESLAE MSIMTTELQS LCSLLQESKE EAIRTLQRKI CELQARLQAQ EEQHQEVQKA KEADIEKLNQ ALCLRYKNEK ELQEVIQQQN EKILEQIDKS GELISLREEV THLTRSLRRA ETETKVLQEA LAGQLDSNCQ PMATNWIQEK VWLSQEVDKL RVMFLEMKNE KEKLMIKFQS HRNILEENLR RSDKELEKLD DIVQHIYKTL LSIPEVVRGC KELQGLLEFL S (844-1193 aa encoded by BC000322)
SPAG5 (sperm associated antigen 5), also known as MAP126, hMAP126, DEEPEST or Astrin, is a 1,193 amino acid protein that is expressed at high levels in testis and at lower levels in liver, placenta, pancreas, colon and thymus. Localized specifically to spindle poles during prophase and throughout the spindle during metaphase and anaphase, SPAG5 is essential for proper spindle formation and is thought to be involved in the dynamic and functional regulation of mitotic spindles. Additionally, SPAG5 plays a role in sister chromatid cohesion and subsequent chromatid separation and, via its association with chromatids, is an important regulator of chromosome integrity. SPAG5 has a long stalk and a globular head domain through which if forms a homodimer that is characterized by aster-like structures. Upon DNA damage, SPAG5 may be phosphorylated by ATR or ATM.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Astrin; Deepest; MAP126; mitotic spindle associated protein p126; mitotic spindle coiled-coil related protein; Mitotic spindle-associated protein p126; Sperm-associated antigen 5
Gene Aliases: DEEPEST; hMAP126; MAP126; SPAG5
UniProt ID: (Human) Q96R06
Entrez Gene ID: (Human) 10615
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