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Invitrogen
SPAG5 Polyclonal Antibody
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FIGURE: 1 / 1
SPAG5 Antibody (PA5-35985) in ICC/IF
SPAG5 Polyclonal Antibody detects SPAG5 protein at spindle by immunofluorescent analysis. Sample: HeLa cells were fixed in 100% MeOH for 5 min. Green: SPAG5 protein stained by SPAG5 Polyclonal Antibody (Product # PA5-35985) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-35985
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human SPAG5. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.84 mg/mL
Amount
84 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Predicted reactivity: Rat (80%), Pig (89%), Bovine (88%).
Target Information
SPAG5 (sperm associated antigen 5), also known as MAP126, hMAP126, DEEPEST or Astrin, is a 1,193 amino acid protein that is expressed at high levels in testis and at lower levels in liver, placenta, pancreas, colon and thymus. Localized specifically to spindle poles during prophase and throughout the spindle during metaphase and anaphase, SPAG5 is essential for proper spindle formation and is thought to be involved in the dynamic and functional regulation of mitotic spindles. Additionally, SPAG5 plays a role in sister chromatid cohesion and subsequent chromatid separation and, via its association with chromatids, is an important regulator of chromosome integrity. SPAG5 has a long stalk and a globular head domain through which if forms a homodimer that is characterized by aster-like structures. Upon DNA damage, SPAG5 may be phosphorylated by ATR or ATM.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Astrin; coiled-coil protein; Deepest; MAP126; mitotic spindle associated protein p126; mitotic spindle coiled-coil related protein; Mitotic spindle-associated protein p126; Sperm-associated antigen 5; unnamed protein product
Gene Aliases: DEEPEST; hMAP126; MAP126; SPAG5
UniProt ID: (Human) Q96R06
Entrez Gene ID: (Human) 10615
mitotic sister chromatid segregation
spindle organization
chromosome segregation
positive regulation of intracellular transport
establishment of spindle orientation
cell division
regulation of attachment of spindle microtubules to kinetochore
protein localization to centrosome
regulation of metaphase plate congression
positive regulation of spindle assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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