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SPATA22 Polyclonal Antibody

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SPATA22 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 16989-1-AP (SPATA22 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

SPATA22 Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SPATA22 Antibody in Immunoprecipitation (IP) — IP result of anti-SPATA22 (IP:16989-1-AP, 3ug; Detection:16989-1-AP 1:500) with mouse testis tissue lysate 4800ug.

Product Details

16989-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SPATA22 Fusion Protein Ag10591 (1-363 aa encoded by BC029483) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.25 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MKRSLNENS ARSTAGCLPV PLFNQKKRNR QPLTSNPLKD DSGISTPSDN YDFPPLPTDW AWEAVNPELA PVMKTVDTGQ IPHSVSRPLR SQDSVFNSIQ SNTGRSQGGW SYRDGNKNTS LKTWNKNDFK PQCKRTNLVA NDGKNSCPMS SGAQQQKQLR TPEPPNLSRN KETELLRQTH SSKISGCTMR GLDKNSALQT LKPNFQQNQY KKQMLDDIPE DNTLKETSLY QLQFKEKASS LRIISAVIES MKYWREHAQK TVLLFEVLAV LDSAVTPGPY YSKTFLMRDG KNTLPCVFYE IDRELPRLIR GRVHRCVGNY DQKKNIFQCV SVRPASVSEQ KTFQAFVKIA DVEMQYYINV MNET (1-363 aa encoded by BC029483)

Target Information

SPATA22 is highly expressed in adult testis. The specific function of SPATA22 is not yet known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination.

Nature communications

Luo M,Yang F,Leu NA,Landaiche J,Handel MA,Benavente R,La Salle S,Wang PJ

Tue Jul 15 00:00:00 EDT 2014

Spata22, a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells.

Biology of reproduction

La Salle S,Palmer K,O'Brien M,Schimenti JC,Eppig J,Handel MA

Wed Feb 01 00:00:00 EST 2012

Spata22, a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells.

Biology of reproduction

La Salle S,Palmer K,O'Brien M,Schimenti JC,Eppig J,Handel MA

Wed Feb 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: Spermatogenesis-associated protein 22; testicular tissue protein Li 186; Testis development protein NYD-SP20

Gene Aliases: Gm882; NYD-SP20; NYDSP20; Repro42; RGD1565378; Sgn; SPATA22

UniProt ID: (Human) Q8NHS9, (Mouse) Q5SV06

Entrez Gene ID: (Human) 84690, (Mouse) 380709, (Rat) 360565

Function(s)

meiotic DNA repair synthesis synapsis gamete generation fertilization meiotic cell cycle regulation of meiotic cell cycle reproductive system development

Process(es)

protein binding

SPATA22 Polyclonal Antibody

Product Details

16989-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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