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Invitrogen
SPC25 Polyclonal Antibody
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FIGURE: 1 / 1
SPC25 Antibody (PA5-120101) in WB
Western blot analysis of SPC25 in extracts of various cell lines. Samples were incubated with SPC25 Polyclonal antibody (Product # PA5-120101) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-120101
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 120-224 of human SPC25 (NP_065726.1).
Conjugate
Form
Liquid
Concentration
0.81 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: HeLa, PC-3. The target is usually found in the following locations: Chromosome, Nucleus, centromere, kinetochore.
Immunogen sequence: ANKANAERLK RLQKSADLYK DRLGLEIRKI YGEKLQFIFT NIDPKNPESP FMFSLHLNEA RDYEVSDSAP HLEGLAEFQE NVRKTNNFSA FLANVRKAFT ATVYN
Target Information
This gene encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2600017H08Rik; hSpc25; Kinetochore protein Spc25; SPC25, NDC80 kinetochore complex component, homolog; spindle pole body component 25 homolog
Gene Aliases: AD024; hSpc25; SPBC25; SPC25
UniProt ID: (Human) Q9HBM1
Entrez Gene ID: (Human) 57405
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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