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Invitrogen
SPDL1 Polyclonal Antibody
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FIGURE: 1 / 2
SPDL1 Antibody (PA5-99285) in ICC/IF
Immunofluorescent analysis of SPDL1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and SPDL1 polyclonal antibody (Product # PA5-99285) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-99285
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SPDL1(Accession Q96EA4), corresponding to amino acid residues S576-Q605.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total CCDC99.
Target Information
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). The protein was found mainly in stratified squamous epithelium. It is moderately repressed by retinoic acid. The antigen localized to basal keratinocytes, although it was also found at lower levels in the suprabasal layers, where it concentrated to areas of cell-to-cell contact. The cellular localization and its striking down-regulation in cultured keratinocytes imply a role in cell-cell and/or cell-matrix interactions necessary for normal growth control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARG1; Arsenite-related gene 1 protein; coiled-coil domain containing 99; Coiled-coil domain-containing protein 99; hSpindly; Protein Spindly; Rhabdomyosarcoma antigen MU-RMS-40.4A; rrhabdomyosarcoma antigen protein MU-RMS-40.4A; Spindle apparatus coiled-coil domain-containing protein 1; unnamed protein product
Gene Aliases: 1700018I02Rik; 2600001J17Rik; 2810049B11Rik; AA409762; CCDC99; SPDL1
UniProt ID: (Human) Q96EA4, (Mouse) Q923A2
Entrez Gene ID: (Human) 54908, (Mouse) 70385
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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