Proteintech

SPHK1 Polyclonal Antibody

SPHK1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

SPHK1 Antibody (10670-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed L02 cells using 10670-1-AP (SPHK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SPHK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SPHK1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10670-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 26 publications 26 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SPHK1 Fusion Protein Ag1051 (85-384 aa encoded by BC008040)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: HEVVNGLME RPDWETAIQK PLCSLPAGSG NALAASLNHY AGYEQVTNED LLTNCTLLLC RRLLSPMNLL SLHTASGLRL FSVLSLAWGF IADVDLESEK YRRLGEMRFT LGTFLLLAAL RTYRGRLAYL PVGRVGSKTP ASPVVVQQGP VDAHLVPLEE PVPSHWTVVP DEDFVLVLAL LHSHLGSEMF AAPMGRCAAG VMHLFYVRAG VSRAMLLRLF LAMEKGRHME YECPYLVYVP VVAFRLEPKD GKGVFAVDGE LMVSEAVQGQ VHPNYFWMVS GCVEPPPSWK PQQMPPPEEP L (85-384 aa encoded by BC008040)

Target Information

SPHK1, along with SPHK2, catalyzes the phosphorylation of sphingosine producing the signaling molecule sphingosine-1-phosphate (S1P). S1P signals through G-protein coupled receptors and controls diverse cellular processes. Inhibitors of SPHKs are viewed as potential therapeutics for the treatment of cancer. SPHK1 catalyzes the phosphorylation of sphingosine to sphingosine 1 phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The preventive effect of mussel oil on gestational diabetes mellitus in pregnant mice fed by a high-fat and high-sucrose diet.

Food & function

Li K,Li H,Song X,Kuang X,Liu S,Zhu S,Li D

Mon Jan 23 00:00:00 EST 2023

Sphingosine Kinases at the Intersection of Pro-Inflammatory LPS and Anti-Inflammatory Endocannabinoid Signaling in BV2 Mouse Microglia Cells.

International journal of molecular sciences

Standoli S,Rapino C,Di Meo C,Rudowski A,Kämpfer-Kolb N,Volk LM,Thomas D,Trautmann S,Schreiber Y,Meyer Zu Heringdorf D,Maccarrone M

Tue May 09 00:00:00 EDT 2023

A novel co-target of ACY1 governing plasma membrane translocation of SphK1 contributes to inflammatory and neuropathic pain.

iScience

Liu B,Wu W,Cui L,Zheng X,Li N,Zhang X,Duan G

Fri Jun 16 00:00:00 EDT 2023

Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer.

Cancer cell international

Zhang E,Ding C,Li S,Aikemu B,Zhou X,Fan X,Sun J,Yang X,Zheng M

Thu May 18 00:00:00 EDT 2023

Ginkgolide B targets and inhibits creatine kinase B to regulate the CCT/TRiC-SK1 axis and exerts pro-angiogenic activity in middle cerebral artery occlusion mice.

Pharmacological research

Zhu J,Jin Z,Yang L,Zhao C,Hu J,Chen J,Han Y,Yu P,Luo J,Kong L,Zhang C

Wed Jun 01 00:00:00 EDT 2022

Metabolomic Analysis Reveals that SPHK1 Promotes Oral Squamous Cell Carcinoma Progression through NF-κB Activation.

Annals of surgical oncology

Hou CX,Mao GY,Sun QW,Meng Y,Zhu QH,Tang YT,Han W,Sun NN,Song XM,Wang CX,Ye JH

Tue Nov 01 00:00:00 EDT 2022

Metabolomic Analysis Reveals that SPHK1 Promotes Oral Squamous Cell Carcinoma Progression through NF-κB Activation.

Annals of surgical oncology

Hou CX,Mao GY,Sun QW,Meng Y,Zhu QH,Tang YT,Han W,Sun NN,Song XM,Wang CX,Ye JH

Tue Nov 01 00:00:00 EDT 2022

LASS2 impairs proliferation of glioma stem cells and migration and invasion of glioma cells mainly via inhibition of EMT and apoptosis promotion.

Journal of Cancer

Zhao WJ,Fan YP,Ou GY,Qiao XY

Sat Jul 16 00:00:00 EDT 2022

LASS2 impairs proliferation of glioma stem cells and migration and invasion of glioma cells mainly via inhibition of EMT and apoptosis promotion.

Journal of Cancer

Zhao WJ,Fan YP,Ou GY,Qiao XY

Sat Jul 16 00:00:00 EDT 2022

LASS2 impairs proliferation of glioma stem cells and migration and invasion of glioma cells mainly via inhibition of EMT and apoptosis promotion.

Journal of Cancer

Zhao WJ,Fan YP,Ou GY,Qiao XY

Sat Jul 16 00:00:00 EDT 2022

Potential of a sphingosine 1-phosphate receptor antagonist and sphingosine kinase inhibitors as targets for multiple myeloma treatment.

Oncology letters

Tanaka Y,Okabe S,Ohyashiki K,Gotoh A

Fri Apr 01 00:00:00 EDT 2022

Berberine ameliorates erectile dysfunction in rats with streptozotocin-induced diabetes mellitus through the attenuation of apoptosis by inhibiting the SPHK1/S1P/S1PR2 and MAPK pathways.

Andrology

Liu K,Sun T,Luan Y,Chen Y,Song J,Ling L,Yuan P,Li R,Cui K,Ruan Y,Lan R,Wang T,Wang S,Liu J,Rao K

Tue Feb 01 00:00:00 EST 2022

Geniposide inhibits SphK1 membrane targeting to restore macrophage polarization balance in collagen-induced arthritis mice.

European journal of pharmacology

Gan PR,Wang RH,Deng R,Wu H,Bu YH,Chen FY,Dong XT,Ke JT

Sat Oct 15 00:00:00 EDT 2022

Proteomic-Based Approach Reveals the Involvement of Apolipoprotein A-I in Related Phenotypes of Autism Spectrum Disorder in the BTBR Mouse Model.

International journal of molecular sciences

Li Q,Shi Y,Li X,Yang Y,Zhang X,Xu L,Ma Z,Wang J,Fan L,Wu L

Sun Dec 04 00:00:00 EST 2022

Identifying the mechanisms and molecular targets of Hongjingtian injection on treatment of TGFβ1-induced HK-2 cells: coupling network pharmacology with experimental verification.

Annals of translational medicine

Sun G,Jiao M,Cui Y,Liang X,Liang X,Zhang S,Guo C

Thu Dec 01 00:00:00 EST 2022

Clonorchis sinensis infection induces hepatobiliary injury via disturbing sphingolipid metabolism and activating sphingosine 1-phosphate receptor 2.

Frontiers in cellular and infection microbiology

Liu JX,Liu M,Yu GZ,Zhao QQ,Wang JL,Sun YH,Koda S,Zhang B,Yu Q,Yan C,Tang RX,Jiang ZH,Zheng KY

Tue Nov 08 00:00:00 EST 2022

Inhibition of Sphingosine-1-Phosphate Receptor 2 Prevents Thoracic Aortic Dissection and Rupture.

Frontiers in cardiovascular medicine

Pan G,Liao M,Dai Y,Li Y,Yan X,Mai W,Liu J,Liao Y,Qiu Z,Zhou Z

Tue Jan 04 00:00:00 EST 2022

SPHK1 contributes to cisplatin resistance in bladder cancer cells via the NONO/STAT3 axis.

International journal of molecular medicine

Qin Z,Tong H,Li T,Cao H,Zhu J,Yin S,He W

Mon Nov 01 00:00:00 EDT 2021

Combined effect of co-exposure to di (2-ethylhexyl) phthalates and 50-Hz magnetic-fields on promoting human amniotic cells proliferation.

Ecotoxicology and environmental safety

Chen L,Ye A,Liu X,Lu J,Xie Q,Guo Y,Sun W

Thu Aug 26 00:00:00 EDT 2021

SphK2/S1P Promotes Metastasis of Triple-Negative Breast Cancer Through the PAK1/LIMK1/Cofilin1 Signaling Pathway.

Frontiers in molecular biosciences

Shi W,Ma D,Cao Y,Hu L,Liu S,Yan D,Zhang S,Zhang G,Wang Z,Wu J,Jiang C

Fri Apr 22 00:00:00 EDT 2022

SphK2/S1P Promotes Metastasis of Triple-Negative Breast Cancer Through the PAK1/LIMK1/Cofilin1 Signaling Pathway.

Frontiers in molecular biosciences

Shi W,Ma D,Cao Y,Hu L,Liu S,Yan D,Zhang S,Zhang G,Wang Z,Wu J,Jiang C

Fri Apr 22 00:00:00 EDT 2022

Bone marrow mesenchymal stem cells-derived exosomes reduce Aβ deposition and improve cognitive function recovery in mice with Alzheimer's disease by activating sphingosine kinase/sphingosine-1-phosphate signaling pathway.

Cell biology international

Wang X,Yang G

Thu Apr 01 00:00:00 EDT 2021

Sphingosine kinase 1 promotes the metastasis of colorectal cancer by inducing the epithelial‑mesenchymal transition mediated by the FAK/AKT/MMPs axis.

International journal of oncology

Liu SQ,Xu CY,Wu WH,Fu ZH,He SW,Qin MB,Huang JA

Tue Jan 01 00:00:00 EST 2019

Sphingosine kinase 1 promotes the metastasis of colorectal cancer by inducing the epithelial‑mesenchymal transition mediated by the FAK/AKT/MMPs axis.

International journal of oncology

Liu SQ,Xu CY,Wu WH,Fu ZH,He SW,Qin MB,Huang JA

Tue Jan 01 00:00:00 EST 2019

Sphingosine kinase 1 promotes the metastasis of colorectal cancer by inducing the epithelial‑mesenchymal transition mediated by the FAK/AKT/MMPs axis.

International journal of oncology

Liu SQ,Xu CY,Wu WH,Fu ZH,He SW,Qin MB,Huang JA

Tue Jan 01 00:00:00 EST 2019

Sphingosine kinase 1 promotes the metastasis of colorectal cancer by inducing the epithelial‑mesenchymal transition mediated by the FAK/AKT/MMPs axis.

International journal of oncology

Liu SQ,Xu CY,Wu WH,Fu ZH,He SW,Qin MB,Huang JA

Tue Jan 01 00:00:00 EST 2019

Sphingosine kinase 1 knockout alleviates hepatic ischemia/reperfusion injury by attenuating inflammation and oxidative stress in mice.

Hepatobiliary & pancreatic diseases international : HBPD INT

Qiang GH,Wang ZX,Ji AL,Wu JY,Cao Y,Zhang G,Zhang YY,Jiang CP

Sat Jun 01 00:00:00 EDT 2019

Decreased S1P and SPHK2 are involved in pancreatic acinar cell injury.

Biomarkers in medicine

Xiao J,Lin H,Liu B,Xia Z,Zhang J,Jin J

Sat Jun 01 00:00:00 EDT 2019

POTEE drives colorectal cancer development via regulating SPHK1/p65 signaling.

Cell death & disease

Shen Z,Feng X,Fang Y,Li Y,Li Z,Zhan Y,Lin M,Li G,Ding Y,Deng H

Wed Nov 13 00:00:00 EST 2019

POTEE drives colorectal cancer development via regulating SPHK1/p65 signaling.

Cell death & disease

Shen Z,Feng X,Fang Y,Li Y,Li Z,Zhan Y,Lin M,Li G,Ding Y,Deng H

Wed Nov 13 00:00:00 EST 2019

Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.

Frontiers in pharmacology

Pang HQ,Yue SJ,Tang YP,Chen YY,Tan YJ,Cao YJ,Shi XQ,Zhou GS,Kang A,Huang SL,Shi YJ,Sun J,Tang ZS,Duan JA

Thu Oct 01 00:00:00 EDT 2020

Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.

Frontiers in pharmacology

Pang HQ,Yue SJ,Tang YP,Chen YY,Tan YJ,Cao YJ,Shi XQ,Zhou GS,Kang A,Huang SL,Shi YJ,Sun J,Tang ZS,Duan JA

Thu Oct 01 00:00:00 EDT 2020

Bidirectional effects of moxifloxacin on the pro‑inflammatory response in lipopolysaccharide‑stimulated mouse peritoneal macrophages.

Molecular medicine reports

Qiu Z,Yuan H,Li N,Yang X,Hu X,Su F,Chen B

Sat Dec 01 00:00:00 EST 2018

Bidirectional effects of moxifloxacin on the pro‑inflammatory response in lipopolysaccharide‑stimulated mouse peritoneal macrophages.

Molecular medicine reports

Qiu Z,Yuan H,Li N,Yang X,Hu X,Su F,Chen B

Sat Dec 01 00:00:00 EST 2018

SphK1 modulates cell migration and EMT-related marker expression by regulating the expression of p-FAK in colorectal cancer cells.

International journal of molecular medicine

Xu CY,Liu SQ,Qin MB,Zhuge CF,Qin L,Qin N,Lai MY,Huang JA

Mon May 01 00:00:00 EDT 2017

SphK1 modulates cell migration and EMT-related marker expression by regulating the expression of p-FAK in colorectal cancer cells.

International journal of molecular medicine

Xu CY,Liu SQ,Qin MB,Zhuge CF,Qin L,Qin N,Lai MY,Huang JA

Mon May 01 00:00:00 EDT 2017

ApoA-I induces S1P release from endothelial cells through ABCA1 and SR-BI in a positive feedback manner.

Journal of physiology and biochemistry

Liu X,Ren K,Suo R,Xiong SL,Zhang QH,Mo ZC,Tang ZL,Jiang Y,Peng XS,Yi GH

Thu Dec 01 00:00:00 EST 2016

ApoA-I induces S1P release from endothelial cells through ABCA1 and SR-BI in a positive feedback manner.

Journal of physiology and biochemistry

Liu X,Ren K,Suo R,Xiong SL,Zhang QH,Mo ZC,Tang ZL,Jiang Y,Peng XS,Yi GH

Thu Dec 01 00:00:00 EST 2016

MiR-506 suppresses liver cancer angiogenesis through targeting sphingosine kinase 1 (SPHK1) mRNA.

Biochemical and biophysical research communications

Lu Z,Zhang W,Gao S,Jiang Q,Xiao Z,Ye L,Zhang X

Mon Mar 21 00:00:00 EDT 2016

MiR-506 suppresses liver cancer angiogenesis through targeting sphingosine kinase 1 (SPHK1) mRNA.

Biochemical and biophysical research communications

Lu Z,Zhang W,Gao S,Jiang Q,Xiao Z,Ye L,Zhang X

Mon Mar 21 00:00:00 EDT 2016

Long non-coding RNA HULC promotes tumor angiogenesis in liver cancer by up-regulating sphingosine kinase 1 (SPHK1).

Oncotarget

Lu Z,Xiao Z,Liu F,Cui M,Li W,Yang Z,Li J,Ye L,Zhang X

Tue Jan 05 00:00:00 EST 2016

Hepatitis B virus X protein promotes human hepatoma cell growth via upregulation of transcription factor AP2α and sphingosine kinase 1.

Acta pharmacologica Sinica

Lu ZP,Xiao ZL,Yang Z,Li J,Feng GX,Chen FQ,Li YH,Feng JY,Gao YE,Ye LH,Zhang XD

Thu Oct 01 00:00:00 EDT 2015

Hepatitis B virus X protein promotes human hepatoma cell growth via upregulation of transcription factor AP2α and sphingosine kinase 1.

Acta pharmacologica Sinica

Lu ZP,Xiao ZL,Yang Z,Li J,Feng GX,Chen FQ,Li YH,Feng JY,Gao YE,Ye LH,Zhang XD

Thu Oct 01 00:00:00 EDT 2015

Hepatitis B virus X protein promotes human hepatoma cell growth via upregulation of transcription factor AP2α and sphingosine kinase 1.

Acta pharmacologica Sinica

Lu ZP,Xiao ZL,Yang Z,Li J,Feng GX,Chen FQ,Li YH,Feng JY,Gao YE,Ye LH,Zhang XD

Thu Oct 01 00:00:00 EDT 2015

Phosphorylation and action of the immunomodulator FTY720 inhibits vascular endothelial cell growth factor-induced vascular permeability.

The Journal of biological chemistry

Sanchez T,Estrada-Hernandez T,Paik JH,Wu MT,Venkataraman K,Brinkmann V,Claffey K,Hla T

Fri Nov 21 00:00:00 EST 2003

Bioinformatics

Protein Aliases: Acetyltransferase SPHK1; RP23-193A16.4; SK 1; SKase; Sphingosine kinase 1; SPK 1

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Gene Aliases: 1110006G24Rik; SK1; SPHK; SPHK1; SPK; Spk1

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UniProt ID: (Human) Q9NYA1, (Rat) Q642F6, (Mouse) Q8CI15

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Entrez Gene ID: (Human) 8877, (Rat) 170897, (Mouse) 20698

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Function(s)

magnesium ion binding DNA binding protein binding calmodulin binding ATP binding sphinganine kinase activity D-erythro-sphingosine kinase activity sphingosine-1-phosphate receptor activity protein phosphatase 2A binding NAD+ kinase activity diacylglycerol kinase activity nucleotide binding kinase activity transferase activity kinase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SPHK1 Polyclonal Antibody

SPHK1 Antibody (10670-1-AP) in ICC/IF

Product Details

10670-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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