Bioss

SPHK1 Polyclonal Antibody

View all (31) SPHK1 antibodies

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Datasheet
Protocols
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Protocols
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Product Details

BS-2652R

Applications
Tested Dilution
Publications

Western Blot (WB)

1:30
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

Flow Cytometry (Flow)

1:20-100
-

ELISA (ELISA)

1:500-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human SPHK1, amino acids 1-100.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

SPHK1, along with SPHK2, catalyzes the phosphorylation of sphingosine producing the signaling molecule sphingosine-1-phosphate (S1P). S1P signals through G-protein coupled receptors and controls diverse cellular processes. Inhibitors of SPHKs are viewed as potential therapeutics for the treatment of cancer. SPHK1 catalyzes the phosphorylation of sphingosine to sphingosine 1 phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Acetyltransferase SPHK1; RP23-193A16.4; SK 1; SKase; Sphingosine kinase 1; SPK 1

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Gene Aliases: 1110006G24Rik; SK1; SPHK; SPHK1; SPK; Spk1

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UniProt ID: (Human) Q9NYA1, (Rat) Q91V26, (Mouse) Q8CI15

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Entrez Gene ID: (Human) 8877, (Rat) 170897, (Mouse) 20698

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Function(s)
magnesium ion binding DNA binding protein binding calmodulin binding ATP binding sphinganine kinase activity D-erythro-sphingosine kinase activity sphingosine-1-phosphate receptor activity protein phosphatase 2A binding NAD+ kinase activity diacylglycerol kinase activity nucleotide binding kinase activity transferase activity kinase
Process(es)
blood vessel development positive regulation of neurotransmitter secretion sphingosine-1-phosphate signaling pathway protein folding sphingosine metabolic process inflammatory response signal transduction brain development female pregnancy cellular response to starvation positive regulation of peptidyl-threonine phosphorylation regulation of tumor necrosis factor-mediated signaling pathway positive regulation of neuron projection development response to amine cyclooxygenase pathway calcium-mediated signaling sphingolipid biosynthetic process positive regulation of cell growth positive regulation of cell migration positive regulation of protein ubiquitination response to magnesium ion response to progesterone regulation of interleukin-1 beta production response to ATP intracellular signal transduction positive regulation of NF-kappaB import into nucleus negative regulation of apoptotic process positive regulation of angiogenesis positive regulation of mitotic cell cycle positive regulation of smooth muscle contraction sphingosine biosynthetic process sphingoid catabolic process lipid phosphorylation positive regulation of fibroblast proliferation positive regulation of NF-kappaB transcription factor activity cellular response to hydrogen peroxide response to interleukin-1 cellular response to growth factor stimulus positive regulation of protein phosphorylation protein kinase C-activating G-protein coupled receptor signaling pathway positive regulation of cell proliferation response to organic cyclic compound phosphorylation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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