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SPHK2 Polyclonal Antibody
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FIGURE: 1 / 3
SPHK2 Antibody (BS-2653R) in ICC/IF
Tissue/cell: HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with SPHK2 Polyclonal Antibody, Unconjugated (bs-2653R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5 µg/mL, blue) was used to stain the cell nuclei.
Product Details
BS-2653R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human SPHK2, amino acids 101-200.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
SPHK2, along with SPHK1, catalyzes the phosphorylation of sphingosine producing the signaling molecule sphingosine-1-phosphate (S1P). S1P signals through G-protein coupled receptors and controls diverse cellular processes. Inhibitors of SPHKs are viewed as potential therapeutics for the treatment of cancer. SPHK2 is an important signaling molecule with intra- and extracellular functions and has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors). These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide raange of cellular events including cell motility, survival, apoptosis, migration and cell-cell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: lipid kinase; SK 2; Sphingosine kinase 2; SPHK2; SPK 2; Spk2, Sk2; unnamed protein product
Gene Aliases: C76851; SK 2; SK-2; SK2; SPHK2; SPK 2; SPK-2
UniProt ID: (Human) Q9NRA0, (Mouse) Q9JIA7
Entrez Gene ID: (Human) 56848, (Rat) 308589, (Mouse) 56632
nucleotide binding
lipid kinase activity
protein binding
ATP binding
sphinganine kinase activity
kinase activity
transferase activity
small GTPase binding
sphingosine-1-phosphate receptor activity
histone binding
NAD+ kinase activity
diacylglycerol kinase activity
D-erythro-sphingosine kinase activity
kinase
blood vessel development
positive regulation of cytokine production involved in immune response
sphingosine-1-phosphate signaling pathway
lipid metabolic process
sphinganine-1-phosphate biosynthetic process
sphingosine metabolic process
brain development
female pregnancy
cell proliferation
positive regulation of cell proliferation
sphingolipid biosynthetic process
negative regulation of cell growth
positive regulation of interleukin-13 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
positive regulation of mast cell activation involved in immune response
regulation of cellular amide metabolic process
intracellular signal transduction
regulation of apoptotic process
positive regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of mast cell degranulation
positive regulation of gene expression, epigenetic
sphingosine biosynthetic process
regulation of lipid biosynthetic process
regulation of transport
regulation of reactive oxygen species biosynthetic process
cellular response to phorbol 13-acetate 12-myristate
regulation of cytochrome-c oxidase activity
positive regulation of ceramide biosynthetic process
regulation of ATP biosynthetic process
protein kinase C-activating G-protein coupled receptor signaling pathway
phosphorylation
negative regulation of apoptotic process
lipid phosphorylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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