Proteintech

SPI1 Polyclonal Antibody

View all (36) PU.1 antibodies

Questions & Answers

Questions & Answers

Questions & Answers

Additional Information:

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Antibody Testing Data (4)

SPI1 Antibody in Immunocytochemistry (ICC/IF)

SPI1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

SPI1 Antibody (55100-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 55100-1-AP (SPI1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SPI1 Antibody in Immunocytochemistry (ICC/IF)

SPI1 Antibody in Western Blot (WB)

SPI1 Antibody in Western Blot (WB)

SPI1 Antibody in Western Blot (WB)

Product Image Gallery

SPI1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 55100-1-AP (SPI1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SPI1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 55100-1-AP (SPI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SPI1 Antibody in Western Blot (WB) — RAW264.7 cells were subjected to SDS PAGE followed by western blot with 55100-1-AP (SPI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SPI1 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 55100-1-AP (SPI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Product Details

55100-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

33 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody is specific to SPI1.

Target Information

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 31 kDa transforming protein; 31 kDa-transforming protein; 31kDa Transforming Protein; hCG_25181; hematopoietic transcription factor PU.1; SFFV proviral integration 1; SFFV proviral integration 1 protein; SPIA; spleen focus forming virus (SFFV) proviral integration oncogene spi1; spleen focus forming virus proviral integration oncogene spi1; Transcription factor PU.1; Transcription Factor spi1

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Gene Aliases: AGM10; Dis-1; Dis1; OF; PU.1; Sfpi-1; SFPI1; SPI-1; SPI-A; SPI1; Tcfpu1; Tfpu.1

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UniProt ID: (Human) P17947, (Mouse) P17433, (Rat) Q6BDS1

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Entrez Gene ID: (Human) 6688, (Mouse) 20375, (Rat) 366126

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transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding RNA binding protein binding histone deacetylase binding sequence-specific DNA binding NFAT protein binding RNA polymerase II sequence-specific DNA binding transcription factor binding STAT family protein binding DNA-binding transcription factor binding protein sequestering activity RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding winged helix/forkhead transcription factor

negative regulation of transcription from RNA polymerase II promoter vasculature development germinal center B cell differentiation follicular B cell differentiation lymphoid progenitor cell differentiation immature B cell differentiation defense response to tumor cell pro-T cell differentiation myeloid leukocyte differentiation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway positive regulation of gene expression negative regulation of gene expression lymphocyte differentiation cell differentiation erythrocyte differentiation macrophage differentiation osteoclast differentiation granulocyte differentiation lipopolysaccharide-mediated signaling pathway somatic stem cell population maintenance TRAIL-activated apoptotic signaling pathway myeloid dendritic cell differentiation negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of neutrophil degranulation negative regulation of MHC class II biosynthetic process response to ethanol positive regulation of B cell differentiation regulation of erythrocyte differentiation positive regulation of gene expression, epigenetic negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter anatomical structure regression interleukin-6-mediated signaling pathway cellular response to ethanol oncogene-induced cell senescence endothelial to hematopoietic transition negative regulation of protein localization to chromatin positive regulation of p38MAPK cascade negative regulation of NIK/NF-kappaB signaling apoptotic process involved in patterning of blood vessels positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of microglial cell mediated cytotoxicity negative regulation of adipose tissue development pericyte cell differentiation positive regulation of antifungal innate immune response regulation of myeloid progenitor cell differentiation positive regulation of myeloid dendritic cell chemotaxis transcription, DNA-templated transcription from RNA polymerase II promoter histone H3 acetylation hypermethylation of CpG island negative regulation of gene expression, epigenetic negative regulation of histone H4 acetylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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