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Invitrogen
SPIB Polyclonal Antibody
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FIGURE: 1 / 1
SPIB Antibody (PA5-121102) in WB
Western blot analysis of SPIB in extracts of Raji cells. Samples were incubated with SPIB Polyclonal antibody (Product # PA5-121102) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-121102
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-262 of human SPIB (NP_003112.2).
Conjugate
Form
Liquid
Concentration
0.75 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: Raji. The target is usually found in the following locations: Cytoplasm, Nucleus.
Immunogen sequence: MLALEAAQLD GPHFSCLYPD GVFYDLDSCK HSSYPDSEGA PDSLWDWTVA PPVPATPYEA FDPAAAAFSH PQAAQLCYEP PTYSPAGNLE LAPSLEAPGP GLPAYPTENF ASQTLVPPAY APYPSPVLSE EEDLPLDSPA LEVSDSESDE ALVAGPEGKG SEAGTRKKLR LYQFLLGLLT RGDMRECVWW VEPGAGVFQF SSKHKELLAR RWGQQKGNRK RMTYQKLARA LRNYAKTGEI RKVKRKLTYQ FDSALLPAVR RA
Target Information
Spi-B is a transcription factor belonging to a subfamily of ETS transcription factors with a conserved ETS domain that mediates specific DNA binding. Spi-B may form homotypic interactions and is known to interact with IRF4, CREBBP, EP300, SPI1/PU.1 related, JUN and TBP. As a sequence specific transcriptional activator it binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Two isoforms of Spi-B are reported. Spi-B is usually expressed in plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells and promotes their development and block viral replication. Spi-B is not expressed in T-cells or granulocytes but may be present in stem cell populations of the liver and B-cells where it helps in B-cell receptor (BCR) signaling, normal B-cell development and antigenic stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Spi-B transcription factor (Spi-1/PU.1 related); Transcription factor Spi-B
Gene Aliases: SPI-B; SPIB
UniProt ID: (Human) Q01892
Entrez Gene ID: (Human) 6689
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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