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SPIN1 Polyclonal Antibody

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Antibody Testing Data (13)

SPIN1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

SPIN1 Antibody (12105-1-AP) in ICC/IF

Immunofluorescent analysis of HEK-293 cells using 12105-1-AP (SPIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SPIN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SPIN1 Antibody in Immunoprecipitation (IP)

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Product Details

12105-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SPIN1 Fusion Protein Ag2750 (1-262 aa encoded by BC013571)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MKTPFGKTP GQRSRADAGH AGVSANMMKK RTSHKKHRSS VGPSKPVSQP RRNIVGCRIQ HGWKEGNGPV TQWKGTVLDQ VPVNPSLYLI KYDGFDCVYG LELNKDERVS ALEVLPDRVA TSRISDAHLA DTMIGKAVEH MFETEDGSKD EWRGMVLARA PVMNTWFYIT YEKDPVLYMY QLLDDYKEGD LRIMPDSNDS PPAEREPGEV VDSLVGKQVE YAKEDGSKRT GMVIHQVEAK PSVYFIKFDD DFHIYVYDLV KTS (1-262 aa encoded by BC013571)

Target Information

SPIN1 (spindlin 1), also known as SPIN or ovarian cancer-related protein (OCR), is a 262 amino acid nuclear protein suggested to play a role in regulation of the cell-cycle during the transition from gamete to embryo. A member of the SPIN/STSY family, SPIN1 localizes to interphase nucleus and mitotic chromosomes, and is modified by phosphorylation in a cell-cycle-dependent fashion. A meiotic spindle-binding protein, SPIN1 overexpression has been shown to cause defects in mitotic spindles, thereby resulting in chromosome instability and potential tumorigenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

UHRF1 is essential for proper cytoplasmic architecture and function of mouse oocytes and derived embryos.

Life science alliance

Uemura S,Maenohara S,Inoue K,Ogonuki N,Matoba S,Ogura A,Kurumizaka M,Yamagata K,Sharif J,Koseki H,Ueda K,Unoki M,Sasaki H

Tue Aug 01 00:00:00 EDT 2023

Epigenetic Control of Cancer Cell Proliferation and Cell Cycle Progression by HNRNPK via Promoting Exon 4 Inclusion of Histone Code Reader SPIN1.

Journal of molecular biology

Li D,Guo J,Jia R

Wed Mar 15 00:00:00 EDT 2023

Epigenetic Control of Cancer Cell Proliferation and Cell Cycle Progression by HNRNPK via Promoting Exon 4 Inclusion of Histone Code Reader SPIN1.

Journal of molecular biology

Li D,Guo J,Jia R

Wed Mar 15 00:00:00 EDT 2023

The microRNA-381(miR-381)/Spindlin1(SPIN1) axis contributes to cell proliferation and invasion of colorectal cancer cells by regulating the Wnt/β-catenin pathway.

Bioengineered

Zhou L,Wang H,Fang Z,Zhong M,He Y,Zou J,Huang S,Li J,Xiang X,Fang Z

Wed Dec 01 00:00:00 EST 2021

Ubiquitin-independent proteasomal degradation of Spindlin-1 by the E3 ligase HACE1 contributes to cell-cell adhesion.

FEBS letters

Palicharla VR,Gupta D,Bhattacharya D,Maddika S

Mon Feb 01 00:00:00 EST 2021

Ubiquitin-independent proteasomal degradation of Spindlin-1 by the E3 ligase HACE1 contributes to cell-cell adhesion.

FEBS letters

Palicharla VR,Gupta D,Bhattacharya D,Maddika S

Mon Feb 01 00:00:00 EST 2021

Structural mechanism of bivalent histone H3K4me3K9me3 recognition by the Spindlin1/C11orf84 complex in rRNA transcription activation.

Nature communications

Du Y,Yan Y,Xie S,Huang H,Wang X,Ng RK,Zhou MM,Qian C

Thu Feb 11 00:00:00 EST 2021

E2F1-activated SPIN1 promotes tumor growth via a MDM2-p21-E2F1 feedback loop in gastric cancer.

Molecular oncology

Lv BB,Ma RR,Chen X,Zhang GH,Song L,Wang SX,Wang YW,Liu HT,Gao P

Thu Oct 01 00:00:00 EDT 2020

E2F1-activated SPIN1 promotes tumor growth via a MDM2-p21-E2F1 feedback loop in gastric cancer.

Molecular oncology

Lv BB,Ma RR,Chen X,Zhang GH,Song L,Wang SX,Wang YW,Liu HT,Gao P

Thu Oct 01 00:00:00 EDT 2020

Soft Substrate Promotes Osteosarcoma Cell Self-Renewal, Differentiation, and Drug Resistance Through miR-29b and Its Target Protein Spin 1.

ACS biomaterials science & engineering

Li S,Bai H,Chen X,Gong S,Xiao J,Li D,Li L,Jiang Y,Li T,Qin X,Yang H,Wu C,You F,Liu Y

Mon Oct 12 00:00:00 EDT 2020

Nucleolar localization signal and histone methylation reader function is required for SPIN1 to promote rRNA gene expression.

Biochemical and biophysical research communications

Zhang X,Zhu G,Su X,Li H,Wu W

Sat Oct 20 00:00:00 EDT 2018

Nucleolar localization signal and histone methylation reader function is required for SPIN1 to promote rRNA gene expression.

Biochemical and biophysical research communications

Zhang X,Zhu G,Su X,Li H,Wu W

Sat Oct 20 00:00:00 EDT 2018

Nucleolar localization signal and histone methylation reader function is required for SPIN1 to promote rRNA gene expression.

Biochemical and biophysical research communications

Zhang X,Zhu G,Su X,Li H,Wu W

Sat Oct 20 00:00:00 EDT 2018

Nucleolar localization signal and histone methylation reader function is required for SPIN1 to promote rRNA gene expression.

Biochemical and biophysical research communications

Zhang X,Zhu G,Su X,Li H,Wu W

Sat Oct 20 00:00:00 EDT 2018

SPIN1 promotes tumorigenesis by blocking the uL18 (universal large ribosomal subunit protein 18)-MDM2-p53 pathway in human cancer.

eLife

Fang Z,Cao B,Liao JM,Deng J,Plummer KD,Liao P,Liu T,Zhang W,Zhang K,Li L,Margolin D,Zeng SX,Xiong J,Lu H

Fri Mar 16 00:00:00 EDT 2018

SPIN1 promotes tumorigenesis by blocking the uL18 (universal large ribosomal subunit protein 18)-MDM2-p53 pathway in human cancer.

eLife

Fang Z,Cao B,Liao JM,Deng J,Plummer KD,Liao P,Liu T,Zhang W,Zhang K,Li L,Margolin D,Zeng SX,Xiong J,Lu H

Fri Mar 16 00:00:00 EDT 2018

The Tudor domain protein Spindlin1 is involved in intrinsic antiviral defense against incoming hepatitis B Virus and herpes simplex virus type 1.

PLoS pathogens

Ducroux A,Benhenda S,Rivière L,Semmes OJ,Benkirane M,Neuveut C

Mon Sep 01 00:00:00 EDT 2014

The Tudor domain protein Spindlin1 is involved in intrinsic antiviral defense against incoming hepatitis B Virus and herpes simplex virus type 1.

PLoS pathogens

Ducroux A,Benhenda S,Rivière L,Semmes OJ,Benkirane M,Neuveut C

Mon Sep 01 00:00:00 EDT 2014

The Tudor domain protein Spindlin1 is involved in intrinsic antiviral defense against incoming hepatitis B Virus and herpes simplex virus type 1.

PLoS pathogens

Ducroux A,Benhenda S,Rivière L,Semmes OJ,Benkirane M,Neuveut C

Mon Sep 01 00:00:00 EDT 2014

The Tudor domain protein Spindlin1 is involved in intrinsic antiviral defense against incoming hepatitis B Virus and herpes simplex virus type 1.

PLoS pathogens

Ducroux A,Benhenda S,Rivière L,Semmes OJ,Benkirane M,Neuveut C

Mon Sep 01 00:00:00 EDT 2014

Nucleolar protein Spindlin1 recognizes H3K4 methylation and stimulates the expression of rRNA genes.

EMBO reports

Wang W,Chen Z,Mao Z,Zhang H,Ding X,Chen S,Zhang X,Xu R,Zhu B

Fri Oct 28 00:00:00 EDT 2011

Nucleolar protein Spindlin1 recognizes H3K4 methylation and stimulates the expression of rRNA genes.

EMBO reports

Wang W,Chen Z,Mao Z,Zhang H,Ding X,Chen S,Zhang X,Xu R,Zhu B

Fri Oct 28 00:00:00 EDT 2011

Nucleolar protein Spindlin1 recognizes H3K4 methylation and stimulates the expression of rRNA genes.

EMBO reports

Wang W,Chen Z,Mao Z,Zhang H,Ding X,Chen S,Zhang X,Xu R,Zhu B

Fri Oct 28 00:00:00 EDT 2011

Nucleolar protein Spindlin1 recognizes H3K4 methylation and stimulates the expression of rRNA genes.

EMBO reports

Wang W,Chen Z,Mao Z,Zhang H,Ding X,Chen S,Zhang X,Xu R,Zhu B

Fri Oct 28 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: 30000 Mr metaphase complex; Ovarian cancer-related protein; Spindlin-1; Spindlin1; SSEC P

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Gene Aliases: OCR; SPIN; SPIN1; TDRD24

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UniProt ID: (Human) Q9Y657, (Mouse) Q61142, (Rat) Q4V8J7

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Entrez Gene ID: (Human) 10927, (Mouse) 20729, (Rat) 361217

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Function(s)

protein binding methylated histone binding DNA binding molecular_function

Process(es)

multicellular organism development gamete generation rRNA transcription Wnt signaling pathway covalent chromatin modification positive regulation of Wnt signaling pathway meiotic cell cycle cell cycle female meiotic division chromatin modification biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SPIN1 Polyclonal Antibody

SPIN1 Antibody (12105-1-AP) in ICC/IF

Product Details

12105-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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