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Invitrogen
SPIRE2 Polyclonal Antibody
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FIGURE: 1 / 1
SPIRE2 Antibody (PA5-120464) in WB
Western blot analysis of SPIRE2 in extracts of various cells. Samples were incubated with SPIRE2 Polyclonal antibody (Product # PA5-120464) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-120464
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human SPIRE2 (NP_115827.1).
Conjugate
Form
Liquid
Concentration
0.76 mg/mL
Amount
76 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: U-87MG, A431, HeLa, mouse liver, mouse brain, mouse testis, rat brain, rat testis. The target is usually found in the following locations: Cell membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle membrane, Peripheral membrane protein, cytoskeleton, cytosol, perinuclear region.
Immunogen sequence: MARAGSCGGA AAGAGRPEPW ELSLEEVLKA YEQPLNEEQA WAVCFQGCRG LRGSPGRRLR DTGDLLLRGD GSVGAREPEA AEPATMVVPL ASSEAQTVQS LGFAIYRALD WGLDESEERE LSPQLERLID LMANNDSEDS GCGAADEGYG GPEEEEEAEG VPRSVRTFAQ AMRLCAARLT DPRGAQAHYQ
Target Information
Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament (PubMed:21620703). Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning and asymmetric cell division during meiosis (PubMed:21620703). Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis (PubMed:21620703). Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (PubMed:26287480). [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein spire homolog 2; Spir-2; spire actin nucleation factor 2; spire family actin nucleation factor 2; spire homolog 2; unnamed protein product
Gene Aliases: BC026502; KIAA1832; Spir-2; SPIR2; SPIRE2
UniProt ID: (Human) Q8WWL2, (Mouse) Q8K1S6
Entrez Gene ID: (Human) 84501, (Rat) 307925, (Mouse) 234857
protein transport
vesicle-mediated transport
actin cytoskeleton organization
cleavage furrow formation
polar body extrusion after meiotic divisions
actin nucleation
intracellular transport
Golgi vesicle transport
establishment of meiotic spindle localization
actin filament network formation
formin-nucleated actin cable assembly
positive regulation of double-strand break repair
transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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