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| Applications | Tested Dilution | Publications |
|---|---|---|
Immunohistochemistry (IHC) |
1:2500-1:5000 | - |
Immunocytochemistry (ICC/IF) |
0.25-2 µg/mL | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant protein corresponding to Human SPIRE2 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.8 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.2, with 40% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2647870 |
Immunogen sequence: SLPCILNACS GDAKSTSCIN LSVTDAGGSA QRPRPRVLLK APTLAEMEEM NTSEEEESPC GEVTLKRDRS FSEHDLAQ
Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 91%.
Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament (PubMed:21620703). Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning and asymmetric cell division during meiosis (PubMed:21620703). Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis (PubMed:21620703). Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (PubMed:26287480). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Protein spire homolog 2; Spir-2; spire actin nucleation factor 2; spire family actin nucleation factor 2; spire homolog 2; spire-type actin nucleation factor 2
Gene Aliases: KIAA1832; Spir-2; SPIR2; SPIRE2
UniProt ID: (Human) Q8WWL2
Entrez Gene ID: (Human) 84501
Molecular Function:
actin or actin-binding cytoskeletal protein
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