Proteintech

SPOP Polyclonal Antibody

Product Image Gallery

SPOP Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 16750-1-AP (SPOP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SPOP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 16750-1-AP ( SPOP Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

SPOP Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 16750-1-AP ( SPOP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

16750-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 20 publications 20 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 2 publications 2 publications
Immunoprecipitation (IP)	Assay-dependent	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SPOP Fusion Protein Ag10215 (1-374 aa encoded by BC003385) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.21 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MSRVPSPPP PAEMSSGPVA ESWCYTQIKV VKFSYMWTIN NFSFCREEMG EVIKSSTFSS GANDKLKWCL RVNPKGLDEE SKDYLSLYLL LVSCPKSEVR AKFKFSILNA KGEETKAMES QRAYRFVQGK DWGFKKFIRR DFLLDEANGL LPDDKLTLFC EVSVVQDSVN ISGQNTMNMV KVPECRLADE LGGLWENSRF TDCCLCVAGQ EFQAHKAILA ARSPVFSAMF EHEMEESKKN RVEINDVEPE VFKEMMCFIY TGKAPNLDKM ADDLLAAADK YALERLKVMC EDALCSNLSV ENAAEILILA DLHSADQLKT QAVDFINYHA SDVLETSGWK SMVVSHPHLV AEAYRSLASA QCPFLGPPRK RLKQS (1-374 aa encoded by BC003385)

Target Information

Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

AMPK Promotes SPOP-Mediated NANOG Degradation to Regulate Prostate Cancer Cell Stemness.

Developmental cell

Wang X,Jin J,Wan F,Zhao L,Chu H,Chen C,Liao G,Liu J,Yu Y,Teng H,Fang L,Jiang C,Pan W,Xie X,Li J,Lu X,Jiang X,Ge X,Ye D,Wang P

Mon Feb 11 00:00:00 EST 2019

SPOP Promotes Nanog Destruction to Suppress Stem Cell Traits and Prostate Cancer Progression.

Developmental cell

Zhang J,Chen M,Zhu Y,Dai X,Dang F,Ren J,Ren S,Shulga YV,Beca F,Gan W,Wu F,Lin YM,Zhou X,DeCaprio JA,Beck AH,Lu KP,Huang J,Zhao C,Sun Y,Gao X,Pandolfi PP,Wei W

Mon Feb 11 00:00:00 EST 2019

The E2F1-miR-520/372/373-SPOP Axis Modulates Progression of Renal Carcinoma.

Cancer research

Ding M,Lu X,Wang C,Zhao Q,Ge J,Xia Q,Wang J,Zen K,Zhang CY,Zhang C

Sat Dec 15 00:00:00 EST 2018

The E2F1-miR-520/372/373-SPOP Axis Modulates Progression of Renal Carcinoma.

Cancer research

Ding M,Lu X,Wang C,Zhao Q,Ge J,Xia Q,Wang J,Zen K,Zhang CY,Zhang C

Sat Dec 15 00:00:00 EST 2018

miR-372 and miR-373 enhance the stemness of colorectal cancer cells by repressing differentiation signaling pathways.

Molecular oncology

Wang LQ,Yu P,Li B,Guo YH,Liang ZR,Zheng LL,Yang JH,Xu H,Liu S,Zheng LS,Zhou H,Qu LH

Thu Nov 01 00:00:00 EDT 2018

SPOP promotes transcriptional expression of DNA repair and replication factors to prevent replication stress and genomic instability.

Nucleic acids research

Hjorth-Jensen K,Maya-Mendoza A,Dalgaard N,Sigurðsson JO,Bartek J,Iglesias-Gato D,Olsen JV,Flores-Morales A

Fri Oct 12 00:00:00 EDT 2018

SPOP promotes transcriptional expression of DNA repair and replication factors to prevent replication stress and genomic instability.

Nucleic acids research

Hjorth-Jensen K,Maya-Mendoza A,Dalgaard N,Sigurðsson JO,Bartek J,Iglesias-Gato D,Olsen JV,Flores-Morales A

Fri Oct 12 00:00:00 EDT 2018

DUB3 Promotes BET Inhibitor Resistance and Cancer Progression by Deubiquitinating BRD4.

Molecular cell

Jin X,Yan Y,Wang D,Ding D,Ma T,Ye Z,Jimenez R,Wang L,Wu H,Huang H

Thu Aug 16 00:00:00 EDT 2018

SPOP promotes ATF2 ubiquitination and degradation to suppress prostate cancer progression.

Journal of experimental & clinical cancer research : CR

Ma J,Chang K,Peng J,Shi Q,Gan H,Gao K,Feng K,Xu F,Zhang H,Dai B,Zhu Y,Shi G,Shen Y,Zhu Y,Qin X,Li Y,Zhang P,Ye D,Wang C

Wed Jul 11 00:00:00 EDT 2018

Speckle-type POZ protein suppresses hepatocellular carcinoma cell migration and invasion via ubiquitin-dependent proteolysis of SUMO1/sentrin specific peptidase 7.

Biochemical and biophysical research communications

Ji P,Liang S,Li P,Xie C,Li J,Zhang K,Zheng X,Feng M,Li Q,Jiao H,Chi X,Zhao W,Zhang S,Wang X

Sat Jul 07 00:00:00 EDT 2018

Speckle-type POZ protein suppresses hepatocellular carcinoma cell migration and invasion via ubiquitin-dependent proteolysis of SUMO1/sentrin specific peptidase 7.

Biochemical and biophysical research communications

Ji P,Liang S,Li P,Xie C,Li J,Zhang K,Zheng X,Feng M,Li Q,Jiao H,Chi X,Zhao W,Zhang S,Wang X

Sat Jul 07 00:00:00 EDT 2018

Dual inhibition of AKT-mTOR and AR signaling by targeting HDAC3 in PTEN- or SPOP-mutated prostate cancer.

EMBO molecular medicine

Yan Y,An J,Yang Y,Wu D,Bai Y,Cao W,Ma L,Chen J,Yu Z,He Y,Jin X,Pan Y,Ma T,Wang S,Hou X,Weroha SJ,Karnes RJ,Zhang J,Westendorf JJ,Wang L,Chen Y,Xu W,Zhu R,Wang D,Huang H

Sun Apr 01 00:00:00 EDT 2018

Cyclin D-CDK4 kinase destabilizes PD-L1 via cullin 3-SPOP to control cancer immune surveillance.

Nature

Zhang J,Bu X,Wang H,Zhu Y,Geng Y,Nihira NT,Tan Y,Ci Y,Wu F,Dai X,Guo J,Huang YH,Fan C,Ren S,Sun Y,Freeman GJ,Sicinski P,Wei W

Thu Jan 04 00:00:00 EST 2018

Cyclin D-CDK4 kinase destabilizes PD-L1 via cullin 3-SPOP to control cancer immune surveillance.

Nature

Zhang J,Bu X,Wang H,Zhu Y,Geng Y,Nihira NT,Tan Y,Ci Y,Wu F,Dai X,Guo J,Huang YH,Fan C,Ren S,Sun Y,Freeman GJ,Sicinski P,Wei W

Thu Jan 04 00:00:00 EST 2018

Identification of SPOP related metabolic pathways in prostate cancer.

Oncotarget

Yan M,Qi H,Li J,Ye G,Shao Y,Li T,Liu J,Piao HL,Xu G

Tue Nov 28 00:00:00 EST 2017

Prostate cancer-associated SPOP mutations confer resistance to BET inhibitors through stabilization of BRD4.

Nature medicine

Dai X,Gan W,Li X,Wang S,Zhang W,Huang L,Liu S,Zhong Q,Guo J,Zhang J,Chen T,Shimizu K,Beca F,Blattner M,Vasudevan D,Buckley DL,Qi J,Buser L,Liu P,Inuzuka H,Beck AH,Wang L,Wild PJ,Garraway LA,Rubin MA,Barbieri CE,Wong KK,Muthuswamy SK,Huang J,Chen Y,Bradner JE,Wei W

Fri Sep 01 00:00:00 EDT 2017

Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation.

Nature medicine

Zhang P,Wang D,Zhao Y,Ren S,Gao K,Ye Z,Wang S,Pan CW,Zhu Y,Yan Y,Yang Y,Wu D,He Y,Zhang J,Lu D,Liu X,Yu L,Zhao S,Li Y,Lin D,Wang Y,Wang L,Chen Y,Sun Y,Wang C,Huang H

Fri Sep 01 00:00:00 EDT 2017

Cullin 3SPOP ubiquitin E3 ligase promotes the poly-ubiquitination and degradation of HDAC6.

Oncotarget

Tan Y,Ci Y,Dai X,Wu F,Guo J,Liu D,North BJ,Huo J,Zhang J

Tue Jul 18 00:00:00 EDT 2017

Dysregulation of INF2-mediated mitochondrial fission in SPOP-mutated prostate cancer.

PLoS genetics

Jin X,Wang J,Gao K,Zhang P,Yao L,Tang Y,Tang L,Ma J,Xiao J,Zhang E,Zhu J,Zhang B,Zhao SM,Li Y,Ren S,Huang H,Yu L,Wang C

Sat Apr 01 00:00:00 EDT 2017

Tumor suppressor SPOP ubiquitinates and degrades EglN2 to compromise growth of prostate cancer cells.

Cancer letters

Zhang L,Peng S,Dai X,Gan W,Nie X,Wei W,Hu G,Guo J

Sat Apr 01 00:00:00 EDT 2017

Prostate cancer-associated mutation in SPOP impairs its ability to target Cdc20 for poly-ubiquitination and degradation.

Cancer letters

Wu F,Dai X,Gan W,Wan L,Li M,Mitsiades N,Wei W,Ding Q,Zhang J

Sat Jan 28 00:00:00 EST 2017

Tumor suppressor SPOP mediates the proteasomal degradation of progesterone receptors (PRs) in breast cancer cells.

American journal of cancer research

Gao K,Jin X,Tang Y,Ma J,Peng J,Yu L,Zhang P,Wang C

Tue Dec 22 00:00:00 EST 2015

Tumor suppressor SPOP mediates the proteasomal degradation of progesterone receptors (PRs) in breast cancer cells.

American journal of cancer research

Gao K,Jin X,Tang Y,Ma J,Peng J,Yu L,Zhang P,Wang C

Tue Dec 22 00:00:00 EST 2015

SPOP Promotes Ubiquitination and Degradation of the ERG Oncoprotein to Suppress Prostate Cancer Progression.

Molecular cell

Gan W,Dai X,Lunardi A,Li Z,Inuzuka H,Liu P,Varmeh S,Zhang J,Cheng L,Sun Y,Asara JM,Beck AH,Huang J,Pandolfi PP,Wei W

Thu Sep 17 00:00:00 EDT 2015

Truncated ERG Oncoproteins from TMPRSS2-ERG Fusions Are Resistant to SPOP-Mediated Proteasome Degradation.

Molecular cell

An J,Ren S,Murphy SJ,Dalangood S,Chang C,Pang X,Cui Y,Wang L,Pan Y,Zhang X,Zhu Y,Wang C,Halling GC,Cheng L,Sukov WR,Karnes RJ,Vasmatzis G,Zhang Q,Zhang J,Cheville JC,Yan J,Sun Y,Huang H

Thu Sep 17 00:00:00 EDT 2015

Truncated ERG Oncoproteins from TMPRSS2-ERG Fusions Are Resistant to SPOP-Mediated Proteasome Degradation.

Molecular cell

An J,Ren S,Murphy SJ,Dalangood S,Chang C,Pang X,Cui Y,Wang L,Pan Y,Zhang X,Zhu Y,Wang C,Halling GC,Cheng L,Sukov WR,Karnes RJ,Vasmatzis G,Zhang Q,Zhang J,Cheville JC,Yan J,Sun Y,Huang H