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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 273-301 amino acids from the C-terminal region of human SPP1|
|Purification||Size-exclusion - Dialysis, Ammonium sulfate precipitation|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
SPP1 binds tightly to hydroxyapatite. The protein appears to form an integral part of the mineralized matrix and probably important to cell-matrix interaction. It acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
BNSP; early T-lymphocyte activation 1; nephropontin; OPN; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); secreted phosphoprotein 1 variant 6; SPP-1; SPP1; SPP1/CALPHA1 fusion; urinary stone protein; uropontin
BNSP; BSPI; ETA-1; OPN; PSEC0156; SPP1