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Invitrogen
SPRED2 Polyclonal Antibody
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FIGURE: 1 / 4
SPRED2 Antibody (PA5-115958) in ICC/IF
Immunocytochemistry analysis of SPRED2 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115958) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115958
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SPRED2(Accession Q7Z698), corresponding to amino acid residues R298-S348.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total SPRED2.
Target Information
SPRED2 is a member of the Sprouty /SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (Nonami et al., 2004 ).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Spred-2; sprouty protein with EVH-1 domain 2, related sequence; sprouty related, EVH1 domain containing 2; sprouty-related, EVH1 domain containing 2; Sprouty-related, EVH1 domain-containing protein 2
Gene Aliases: C79158; Spred-2; SPRED2
UniProt ID: (Human) Q7Z698, (Mouse) Q924S7, (Rat) Q3C2P8
Entrez Gene ID: (Human) 200734, (Mouse) 114716, (Rat) 305539
inactivation of MAPK activity
multicellular organism development
fibroblast growth factor receptor signaling pathway
negative regulation of peptidyl-threonine phosphorylation
positive regulation of DNA damage response, signal transduction by p53 class mediator
regulation of protein deacetylation
regulation of signal transduction
negative regulation of protein serine/threonine kinase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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