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          • Primary Antibodies ›
          • SPSA1 Antibodies

          Agrisera

          SPSA1/Sucrose phosphate synthase isoform A1 Polyclonal Antibody

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          Cite SPSA1/Sucrose phosphate synthase isoform A1 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          SPSA1/Sucrose phosphate synthase isoform A1 Antibody in Western Blot (WB)
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          SPSA1/Sucrose phosphate synthase isoform A1 Antibody in Western Blot (WB)
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          SPSA1/Sucrose phosphate synthase isoform A1 Antibody (AS15-2995) in WB

          Proteins were isolated from Arabidopsis thaliana wild-type and SPS mutants were solubilized with 3 X LB (6 M urea, 12% SDS, 30% glycerol, 100 m M DTT, 150 m M Tris p H 7.0,0.8% Comassie G-250). 12 µg of proteins were loaded into each lane and separated on 8% SDS-PAGE, and then blotted overnight onto PVDF membrane. Membranes were blocked with milk powder for 2 h and then incubated in the primary antibody sol... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SPSA1/Sucrose phosphate synthase isoform A1 Antibody in Western Blot (WB)

          Product Details

          AS15-2995

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -
          Product Specifications

          Species Reactivity

          Plant

          Host/Isotype

          Rabbit

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPSA1 sequence.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Amount

          50 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4

          Contains

          no preservative

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

          For reconstitution add 50 µL of sterile water.

          Specific Species Reactivity: Arabidopsis thaliana

          Target Information

          SPSA1, also called sucrose phosphate synthase A1, is an enzyme that catalyzes formation of sucrose 6 phosphate from UDP glucose and fructose 6 phosphate, a key step in sucrose biosynthesis in plants. It helps control carbon partitioning from photosynthetic products toward transportable and storage sugars, influencing growth, stress tolerance, and sink source balance. SPSA1 activity is regulated by allosteric effectors, reversible phosphorylation, and redox status, responding to light, osmotic stress, and hormones to adjust sucrose production. Expression is strong in photosynthetic tissues and developing sinks, and perturbation alters sugar levels, osmotic balance, and metabolism, impacting traits such as yield, stress resilience, and diurnal carbohydrate dynamics.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: AT5G20280; ATSPS1F; F5O24.170; F5O24_170; SPS1; SPS1F; sucrose phosphate synthase 1F; sucrose phosphate synthase isoform A1; sucrose-phosphate synthase A1

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          It has to be done as per old AB suggested Products section.

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