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Invitrogen
SQSTM1 Polyclonal Antibody
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FIGURE: 1 / 15
SQSTM1 Antibody (PA5-27247) in ICC/IF
SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: SQSTM1 protein stained by SQSTM1 antibody [N3C1], Internal (Product # PA5-27247) diluted at 1:1,000. Red: Phalloidin, a F-actin marker.
Product Details
PA5-27247
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Insect,
Mouse,
Rat,
Zebrafish
Published species
Human,
Monkey,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SQSTM1/P62. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.39 mg/mL
Amount
39 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: A549, HepG2, HepG2 (3µM Thapsigargin treatment for 24 hr), Huh7, Huh7 ( 3µM Thapsigargin, 12hr), NIH-3T3, JC, BCL-1, PC-12, Rat2, HeLa.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: autophagy receptor p62; EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; oxidative stress induced; oxidative stress induced like; p62; p62 transcript variant 4; p62 transcript variant 5; phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; PKC-zeta-interacting protein; Protein kinase C-zeta-interacting protein; Sequestosome-1; STONE14; Ubiquitin-binding protein p62; unnamed protein product
Gene Aliases: A170; DMRV; EBIAP; FTDALS3; NADGP; ORCA; OSF-6; Osi; OSIL; p60; p62; p62B; PDB3; sb:cb621; SQSTM1; STAP; STONE14; zgc:85784; ZIP; ZIP3
UniProt ID: (Human) Q13501, (Rat) O08623, (Mouse) Q64337
Entrez Gene ID: (Bovine) 338045, (Human) 8878, (Rat) 113894, (Mouse) 18412, (Zebrafish) 406452
protein kinase C binding
protein binding
zinc ion binding
enzyme binding
protein kinase binding
protein binding, bridging
receptor tyrosine kinase binding
ubiquitin protein ligase binding
ionotropic glutamate receptor binding
signaling receptor activity
SH2 domain binding
identical protein binding
ubiquitin binding
macromolecular complex binding
metal ion binding
K63-linked polyubiquitin binding
ubiquitin-dependent protein binding
protein sequestering activity
molecular sequestering activity
molecular condensate scaffold activity
protein homodimerization activity
transcription cofactor activity
positive regulation of protein phosphorylation
immune system process
ubiquitin-dependent protein catabolic process
autophagy
apoptotic process
macroautophagy
positive regulation of macroautophagy
cell differentiation
regulation of I-kappaB kinase/NF-kappaB signaling
protein heterooligomerization
regulation of mitophagy
mitophagy
mitochondrion organization
endosome organization
axonogenesis
locomotory behavior
regulation of autophagy
aggrephagy
defense response to bacterium
negative regulation of transcription from RNA polymerase II promoter
mitophagy
pexophagy
temperature homeostasis
response to ischemia
transcription from RNA polymerase II promoter
protein import into nucleus
protein localization
positive regulation of autophagy
regulation of mitochondrion organization
endosomal transport
protein catabolic process
negative regulation of protein ubiquitination
cellular response to stress
toll-like receptor 4 signaling pathway
negative regulation of toll-like receptor 4 signaling pathway
intracellular signal transduction
positive regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
regulation of Ras protein signal transduction
regulation of protein complex stability
brown fat cell proliferation
protein K63-linked ubiquitination
protein targeting to vacuole involved in autophagy
energy homeostasis
response to mitochondrial depolarisation
negative regulation of ferroptosis
membraneless organelle assembly
positive regulation of long-term synaptic potentiation
positive regulation of protein localization to plasma membrane
protein localization to perinuclear region of cytoplasm
mitophagy in response to mitochondrial depolarization
regulation of nucleic acid-templated transcription
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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