Proteintech

SRA1 Polyclonal Antibody

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SRA1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed MCF-7 cells using 24655-1-AP (SRA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SRA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 24655-1-AP ( SRA1 Antibody) at dilution of 1:50 (under 40x lens).

SRA1 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 24655-1-AP (SRA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SRA1 Antibody in Flow Cytometry (Flow) — 1X10^6 MCF-7 cells were stained with 0.2ug SRA1 antibody (24655-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Product Details

24655-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SRA1 Fusion Protein Ag20082 (47-236 aa encoded by BC067895)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.51 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This polyclonal antibody recognizes endogenous SRA1 isoforms which migrate as a doublet on SDS-PAGE gels.

Immunogen sequence: GNKERGWND PPQFSYGLQT QAGGPRRSLL TKRVAAPQDG SPRVPASETS PGPPPMGPPP PSSKAPRSPP VGSGPASGVE PTSFPVESEA VMEDVLRPLE QALEDCRGHT RKQVCDDISR RLALLQEQWA GGKLSIPVKK RMALLVQELS SHRWDAADDI HRSLMVDHVT EVSQWMVGVK RLIAEKRSLF (47-236 aa encoded by BC067895)

Target Information

This gene is involved in transcriptional coactivation by steroid receptors. There is currently data suggesting this gene encodes both a non-coding RNA that functions as part of a ribonucleoprotein complex and a protein coding mRNA. Increased expression of both the transcript and the protein is associated with cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Magnetic resonance imaging of tumor-associated-macrophages (TAMs) with a nanoparticle contrast agent.

RSC advances

Zhou J,Meli VS,Yu-Tin Chen E,Kapre R,Nagalla R,Xiao W,Borowsky AD,Lam KS,Liu WF,Louie AY

Tue Mar 08 00:00:00 EST 2022

Effect of Si-Miao-Yong-An decoction on the differentiation of monocytes, macrophages, and regulatory T cells in ApoE^-/- mice.

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Up-regulation of thioredoxin system by puerarin inhibits lipid uptake in macrophages.

Free radical biology & medicine

Li W,Xu X,Dong D,Lei T,Ou H

Fri Jan 01 00:00:00 EST 2021

Dihydrocapsaicin Attenuates Plaque Formation through a PPARγ/LXRα Pathway in apoE(-/-) Mice Fed a High-Fat/High-Cholesterol Diet.

PloS one

Hu YW,Ma X,Huang JL,Mao XR,Yang JY,Zhao JY,Li SF,Qiu YR,Yang J,Zheng L,Wang Q

Thu Oct 12 00:00:00 EDT 2017

Dihydrocapsaicin Attenuates Plaque Formation through a PPARγ/LXRα Pathway in apoE(-/-) Mice Fed a High-Fat/High-Cholesterol Diet.

PloS one

Hu YW,Ma X,Huang JL,Mao XR,Yang JY,Zhao JY,Li SF,Qiu YR,Yang J,Zheng L,Wang Q

Thu Oct 12 00:00:00 EDT 2017

VNN1 promotes atherosclerosis progression in apoE-/- mice fed a high-fat/high-cholesterol diet.

Journal of lipid research

Hu YW,Wu SG,Zhao JJ,Ma X,Lu JB,Xiu JC,Zhang Y,Huang C,Qiu YR,Sha YH,Gao JJ,Wang YC,Li SF,Zhao JY,Zheng L,Wang Q

Mon Aug 01 00:00:00 EDT 2016

VNN1 promotes atherosclerosis progression in apoE-/- mice fed a high-fat/high-cholesterol diet.

Journal of lipid research

Hu YW,Wu SG,Zhao JJ,Ma X,Lu JB,Xiu JC,Zhang Y,Huang C,Qiu YR,Sha YH,Gao JJ,Wang YC,Li SF,Zhao JY,Zheng L,Wang Q

Mon Aug 01 00:00:00 EDT 2016

RP5-833A20.1/miR-382-5p/NFIA-dependent signal transduction pathway contributes to the regulation of cholesterol homeostasis and inflammatory reaction.

Arteriosclerosis, thrombosis, and vascular biology

Hu YW,Zhao JY,Li SF,Huang JL,Qiu YR,Ma X,Wu SG,Chen ZP,Hu YR,Yang JY,Wang YC,Gao JJ,Sha YH,Zheng L,Wang Q

Thu Jan 01 00:00:00 EST 2015

RP5-833A20.1/miR-382-5p/NFIA-dependent signal transduction pathway contributes to the regulation of cholesterol homeostasis and inflammatory reaction.

Arteriosclerosis, thrombosis, and vascular biology

Hu YW,Zhao JY,Li SF,Huang JL,Qiu YR,Ma X,Wu SG,Chen ZP,Hu YR,Yang JY,Wang YC,Gao JJ,Sha YH,Zheng L,Wang Q

Thu Jan 01 00:00:00 EST 2015

RP5-833A20.1/miR-382-5p/NFIA-dependent signal transduction pathway contributes to the regulation of cholesterol homeostasis and inflammatory reaction.

Arteriosclerosis, thrombosis, and vascular biology

Hu YW,Zhao JY,Li SF,Huang JL,Qiu YR,Ma X,Wu SG,Chen ZP,Hu YR,Yang JY,Wang YC,Gao JJ,Sha YH,Zheng L,Wang Q

Thu Jan 01 00:00:00 EST 2015

A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis.

Journal of lipid research

Hu YW,Yang JY,Ma X,Chen ZP,Hu YR,Zhao JY,Li SF,Qiu YR,Lu JB,Wang YC,Gao JJ,Sha YH,Zheng L,Wang Q

Tue Apr 01 00:00:00 EDT 2014

A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis.

Journal of lipid research

Hu YW,Yang JY,Ma X,Chen ZP,Hu YR,Zhao JY,Li SF,Qiu YR,Lu JB,Wang YC,Gao JJ,Sha YH,Zheng L,Wang Q

Tue Apr 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: MGC87674; SRA1; SRAP; steroid receptor coactivator; Steroid receptor RNA activator 1; steroid receptor RNA activator 1 (complexes with NCOA1); Steroid receptor RNA activator protein

Gene Aliases: PP7684; SRA; SRA1; SRAP; STRAA1

UniProt ID: (Human) Q9HD15

Entrez Gene ID: (Human) 10011

Function(s)

transcription, DNA-templated regulation of transcription, DNA-templated apoptotic process cell proliferation cell differentiation regulation of apoptotic process positive regulation of receptor activity

Process(es)

transcription coactivator activity protein binding ligand-dependent nuclear receptor binding ligand-dependent nuclear receptor transcription coactivator activity

SRA1 Polyclonal Antibody

Product Details

24655-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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