Immunofluorescence analysis of SRC1 was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SRC1 Rabbit Polyclonal Antibody (PA1840) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Avian, Human, Mouse|
|Published species reactivity||Avian, Rat, Amphibian, Human, Mouse, Chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues S(8) S D P A N P D S H K R K G S P C(24) of human SRC-1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||1-2 µg/ml|
|Immunofluorescence (IF)||1-2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-840 detects steroid receptor coactivator-1 (SRC-1) from human, mouse, and japaneese quail tissues and cells.
PA1-840 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~165 kDa protein representing SRC-1 and a smaller, unknown protein at ~40 kDa from HeLa cell lysate. Immunocytochemical staining of SRC-1 in HeLa cells with PA1-840 results in nuclear staining.
The PA1-840 immunogen is a synthetic peptide corresponding to residues S(8) S D P A N P D S H K R K G S P C(24) of human SRC-1. This immunizing peptide (Cat. # PEP-044) is available for use in neutralization and control experiments.
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is Steroid Receptor Coactivator-1 (SRC-1).
SRC-1 has been shown to interact with and stimulate the transcriptional activity of estrogen, progesterone, retinoic acid, thyroid and glucocorticoid receptors, as well as retinoic X receptor (RXR) in a ligand dependent manner. The amino terminal region of SRC-1 contains a PAS-A-basic helix-loop-helix homology domain which has previously been shown to be dimerization domains in other nuclear transcription factors including the aryl hydrocarbon (Ah) receptor and its heterodimerization partner, ARNT. The carboxy-terminal region of SRC-1 has been shown to possess histone acetyltransferase activity specific primarily for histones H3 and H4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Seasonal and sexual dimorphisms in expression of androgen receptor and its coactivators in brain and peripheral copulatory tissues of the green anole.
PA1-840 was used in western blot to study the role of the androgen receptor and its co-activators in regulating the seasonal breeding of the green anole lizzard
|Kerver HN,Wade J||General and comparative endocrinology (193:56)||2013|
Electromagnetic fields alter the expression of estrogen receptor cofactors in breast cancer cells.
PA1-840 was used in western blot to investigate the effect of electromagnetic fields on expression of estrogen receptor cofactors in MCF-7 cells
|Girgert R,Gründker C,Emons G,Hanf V||Bioelectromagnetics (29:169)||2008|
Targeting steroid receptor coactivator-1 expression with locked nucleic acids antisense reveals different thresholds for the hormonal regulation of male sexual behavior in relation to aromatase activity and protein expression.
PA1-840 was used in western blot to investigate the association between male sexual behavior and aromatase activity and protein expression
|Charlier TD,Harada N,Ball GF,Balthazart J||Behavioural brain research (172:333)||2006|
Plasticity in the expression of the steroid receptor coactivator 1 in the Japanese quail brain: effect of sex, testosterone, stress and time of the day.
PA1-840 was used in western blot to investigate the regulation of steroid receptor coactivator 1 expression in Japanese quail brain
|Charlier TD,Ball GF,Balthazart J||Neuroscience (140:1381)||2006|
Inhibition of steroid receptor coactivator-1 blocks estrogen and androgen action on male sex behavior and associated brain plasticity.
PA1-840 was used in western blot to investigate the effect of steroid receptor coactivator-1 on steroid-dependent gene transcription and male sex behavior.
|Charlier TD,Ball GF,Balthazart J||The Journal of neuroscience : the official journal of the Society for Neuroscience (25:906)||2005|
Factor recruitment and TIF2/GRIP1 corepressor activity at a collagenase-3 response element that mediates regulation by phorbol esters and hormones.
PA1-840 was used in western blot to investigate the role of TIF2/GRIP1 and its associated receptor on specific transcriptional regulation
|Rogatsky I,Zarember KA,Yamamoto KR||The EMBO journal (20:6071)||2001|
Distribution and sexually dimorphic expression of steroid receptor coactivator-1 (SRC-1) in the zebra finch brain.
PA1-840 was used in immunohistochemistry to identify the effect of steroid hormone levels on SRC-1 expression
|Duncan KA,Jimenez P,Carruth LL||General and comparative endocrinology (170:408)||2011|
Screening of genetic and expression alterations of SRC1 gene in prostate cancer.
PA1-840 was used in immunohistochemistry to investigate SRC1 mutations in prostate cancer
|Mäki HE,Waltering KK,Wallén MJ,Martikainen PM,Tammela TL,van Weerden WM,Vessella RL,Visakorpi T||The Prostate (66:1391)||2006|
Expression of nuclear receptor coactivators in androgen-responsive and -unresponsive motoneurons.
PA1-840 was used in immunohistochemistry to study nuclear receptor coactivators in rat lumbar motoneurons
|O'Bryant EL,Jordan CL||Hormones and behavior (47:29)||2005|
Steroid receptor coactivator-1 is not required for androgen-mediated sexual differentiation of spinal motoneurons.
PA1-840 was used in immunohistochemistry to investigate the association between steroid receptor coactivator-1 and a sexually dimorphic neuromuscular system
|Monks DA,Xu J,O'Malley BW,Jordan CL||Neuroendocrinology (78:45)||2003|
The p160 coactivator PAS-B motif stabilizes nuclear receptor binding and contributes to isoform-specific regulation by thyroid hormone receptors.
PA1-840 was used in immunoprecipitation to investigate the relationship between p160 coactivators and thyroid hormone receptors.
|Privalsky ML,Lee S,Hahm JB,Young BM,Fong RN,Chan IH||The Journal of biological chemistry (284:19554)||2009|