Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1 µg/mL | View 8 publications 8 publications |
Immunohistochemistry (IHC) |
- | View 4 publications 4 publications |
Immunohistochemistry (Frozen) (IHC (F)) |
1 µg/mL | - |
Immunocytochemistry (ICC/IF) |
1-2 µg/mL | - |
Immunoprecipitation (IP) |
- | View 1 publication 1 publication |
ChIP assay (ChIP) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Avian, Human, Mouse |
Published species |
Amphibian, Avian, Chicken, Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthetic peptide corresponding to residues S(8) S D P A N P D S H K R K G S P C(24) of human SRC-1. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS with 1mg/mL BSA |
Contains |
0.05% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2251288 |
PA1-840 detects steroid receptor coactivator-1 (SRC-1) from human, mouse, and japaneese quail tissues and cells.
PA1-840 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~165 kDa protein representing SRC-1 and a smaller, unknown protein at ~40 kDa from HeLa cell lysate. Immunocytochemical staining of SRC-1 in HeLa cells with PA1-840 results in nuclear staining.
The PA1-840 immunogen is a synthetic peptide corresponding to residues S(8) S D P A N P D S H K R K G S P C(24) of human SRC-1. This immunizing peptide (Cat. # PEP-044) is available for use in neutralization and control experiments.
Clone 1135 recognizes SRC-1, steroid-receptor coactivator-1. SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160 kDa and has a basic helix-loop-helix (bHLH)-PAS domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ASV; bHLHe74; c-SRC; Class E basic helix-loop-helix protein 74; Hin-2 protein; mNRC-1; NCoA-1; Nuclear receptor coactivator 1; Nuclear receptor coactivator protein 1; PAX3/NCOA1 fusion protein; Protein Hin-2; Renal carcinoma antigen NY-REN-52; RIP160; SRC-1; SRC-1 (-Q); SRC-a/NCoA-1; Steroid receptor coactivator 1; steroid receptor coactivator-1
Gene Aliases: bHLHe42; BHLHE74; F-SRC-1; KAT13A; NCOA1; RIP160; SRC-1; SRC1
UniProt ID: (Human) Q15788, (Mouse) P70365
Entrez Gene ID: (Human) 8648, (Mouse) 17977
Molecular Function:
histone modifying enzyme
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