Invitrogen

SRC2 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

SRC2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of GRIP1 was done on 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GRIP1 Rabbit Polyclonal Antibody (Product # PA1-846) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

SRC2 Antibody in Western Blot, ChIP assay (WB, ChIP) — Published figure using SRC2 polyclonal antibody (Product # PA1-846) in Western Blot and ChIP assay

SRC2 Antibody in ChIP assay (ChIP) — Published figure using SRC2 polyclonal antibody (Product # PA1-846) in ChIP assay

Product Details

PA1-846

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 4 publications 4 publications
Immunocytochemistry (ICC/IF)	1-2 µg/mL	-
ChIP assay (ChIP)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Hamster, Human
Published species	Human, Non-human primate, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues P(206) L P D S E E E G H D S(217) C of human GRIP1.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2112760

Product Specific Information

PA1-846 detects glucocorticoid receptor interacting protein-1 (GRIP1) from hamster and human cells.

PA1-846 has been successfully used in immunocytochemistry, and immunofluorescence procedures. Immunocytochemical staining of GRIP1 in CHO cells with PA1-846 results in cytoplasmic and nuclear staining. This antibody has not been used successfully in Western blot procedures.

PA1-846 immunogen is a synthetic peptide corresponding to residues P(206) L P D S E E E G H D S(217) C of human GRIP1. The PA1-846 immunizing peptide (Cat. # PEP-050) is available for use in neutralization and control experiments.

Target Information

SRC2 (steroid receptor coactivator 2), also known as nuclear receptor coactivator2, NCOA2 or TIF2, aids in the function of nuclear hormone receptors. Nuclear hormone receptors are conditional transcription factors that play important roles in various aspects of cell growth, development, and homeostasis by controlling expression of specific genes. Members of the nuclear hormone receptor superfamily, which includes the 5 steroid receptors and class II nuclear receptors, are structurally characterized by 3 distinct domains: an N-terminal transcriptional activation domain, a central DNA-binding domain, and a C-terminal hormone-binding domain. Before the binding of hormone, steroid receptors, which are sometimes called class I of the nuclear hormone receptor family, remain inactive in a complex with heat-shock protein-90 and other stress family proteins. Binding of hormone induces critical conformational changes in steroid receptors that cause them to dissociate from the inhibitory complex, bind as homodimers to specific DNA enhancer elements associated with target genes, and modulate that gene's transcription. After binding to enhancer elements, transcription factors require transcriptional coactivator proteins to mediate their stimulation of transcription initiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Regulation of GRIP1 and CBP Coactivator activity by Rho GDI modulates estrogen receptor transcriptional enhancement.

The Journal of biological chemistry

Su LF,Wang Z,Garabedian MJ

PA1-846 was used in western blot to investigate the regulatory mechanism for GRIP1 and CBP coactivator activity.

Fri Oct 04 00:00:00 EDT 2002

Factor recruitment and TIF2/GRIP1 corepressor activity at a collagenase-3 response element that mediates regulation by phorbol esters and hormones.

The EMBO journal

Rogatsky I,Zarember KA,Yamamoto KR

PA1-846 was used in immunoprecipitation and western blot to investigate the role of TIF2/GRIP1 and its associated receptor on specific transcriptional regulation

Thu Nov 01 00:00:00 EST 2001

Factor recruitment and TIF2/GRIP1 corepressor activity at a collagenase-3 response element that mediates regulation by phorbol esters and hormones.

The EMBO journal

Rogatsky I,Zarember KA,Yamamoto KR

PA1-846 was used in immunoprecipitation and western blot to investigate the role of TIF2/GRIP1 and its associated receptor on specific transcriptional regulation

Thu Nov 01 00:00:00 EST 2001

Multiple receptor interaction domains of GRIP1 function in synergy.

Nucleic acids research

Schmidt S,Baniahmad A,Eggert M,Schneider S,Renkawitz R

PA1-846 was used in western blot to study the mechanism for the effect of nuclear hormone receptors on gene transcription

Sun Mar 01 00:00:00 EST 1998

GRIP1, a transcriptional coactivator for the AF-2 transactivation domain of steroid, thyroid, retinoid, and vitamin D receptors.

Molecular and cellular biology

Hong H,Kohli K,Garabedian MJ,Stallcup MR

PA1-846 was used in western blot to investigate the important role of GRIP1 as a transcriptional coactivator interacting with the hormone binding domain of nuclear hormone receptors

Thu May 01 00:00:00 EDT 1997

Bioinformatics

Protein Aliases: bHLHe75; c-fgr; c-src2; Class E basic helix-loop-helix protein 75; FLJ43153; glucocorticoid receptor-interacting protein-1; hTIF2; MGC75096; NCoA-2; Nuclear receptor coactivator 2; p160 steroid receptor coactivator 2; p55c-fgr; p58c-fgr; TIF 2; Transcriptional intermediary factor 2

Gene Aliases: BHLHE75; GRIP1; I79_019839; KAT13C; NCoA-2; NCOA2; SRC2; TIF2

UniProt ID: (Human) Q15596

Entrez Gene ID: (Human) 10499, (Chinese hamster) 100762955

Molecular Function: histone modifying enzyme