PA1-846 detects glucocorticoid receptor interacting protein-1 (GRIP1) from hamster and human cells.
PA1-846 has been successfully used in immunocytochemistry, and immunofluorescence procedures. Immunocytochemical staining of GRIP1 in CHO cells with PA1-846 results in cytoplasmic and nuclear staining. This antibody has not been used successfully in Western blot procedures.
PA1-846 immunogen is a synthetic peptide corresponding to residues P(206) L P D S E E E G H D S(217) C of human GRIP1. The PA1-846 immunizing peptide (Cat. # PEP-050) is available for use in neutralization and control experiments.
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Glucocorticoid receptor interacting protein-1 (GRIP1), or Transcription Intermediary Factor 2 (TIF2), is a member of a family of transcriptional coactivator proteins which includes steroid receptor coactivator-1 (SRC-1) and Amplified in breast cancer-1 (AIB-1).
GRIP1 has been shown to interact and stimulate transcriptional activity of the retinoic acid, retinoid X, vitamin D, mineralocorticoid, glucocorticoid, thyroid hormone, androgen, estrogen, progesterone, and peroxisome proliferator activated receptors. GRIP1 also contains several copies of the conserved LXXLL/LLXXL motif which has been demonstrated to be critical to receptor-coactivator interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bHLHe75; Class E basic helix-loop-helix protein 75; Glucocorticoid Receptor Interacting Protein; glucocorticoid receptor-interacting protein-1; hTIF2; NCoA-2; Nuclear receptor coactivator 2; p160 steroid receptor coactivator 2; TIF 2; Transcription Intermediary Factor 2; Transcriptional intermediary factor 2
Gene Aliases: BHLHE75; GRIP1; I79_019839; KAT13C; NCoA-2; NCOA2; SRC2; TIF2
UniProt ID: (Human) Q15596