Western blot analysis of human SRC2 in nuclear (A) or cytosolic (S100) (B) extracts of Hela cells using a SRC2 polyclonal antibody (Product # PA1-86392) at 20mcg/10mg lysate. Immunoprecipitate was resolved by SDS-PAGE and the identity of the SRC2 band verified by mass spectroscopy
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||citric acid|
|Contains||0.1% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||1-4 ug/mg of lysate|
|Western Blot (WB)||1:1,000 - 1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Predicted to react with mouse based on sequence homology. Does not react with Xenopus.
SRC2 (steroid receptor coactivator 2), also known as nuclear receptor coactivator2, NCOA2 or TIF2, aids in the function of nuclear hormone receptors. Nuclear hormone receptors are conditional transcription factors that play important roles in various aspects of cell growth, development, and homeostasis by controlling expression of specific genes. Members of the nuclear hormone receptor superfamily, which includes the 5 steroid receptors and class II nuclear receptors (see below), are structurally characterized by 3 distinct domains: an N-terminal transcriptional activation domain, a central DNA-binding domain, and a C-terminal hormone-binding domain. Before the binding of hormone, steroid receptors, which are sometimes called class I of the nuclear hormone receptor family, remain inactive in a complex with heat-shock protein-90 (MIM 140571) and other stress family proteins. Binding of hormone induces critical conformational changes in steroid receptors that cause them to dissociate from the inhibitory complex, bind as homodimers to specific DNA enhancer elements associated with target genes, and modulate that gene's transcription. After binding to enhancer elements, transcription factors require transcriptional coactivator proteins to mediate their stimulation of transcription initiation (Hong et al., 1997 [PubMed 9111344]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.