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Invitrogen
SRC2 Polyclonal Antibody
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FIGURE: 1 / 5
SRC2 Antibody (PA5-29482) in ICC/IF
Immunofluorescence analysis of SRC2 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SRC2 Polyclonal Antibody (Product # PA5-29482) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # ... View More
Product Details
PA5-29482
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human NCOA2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HeLa Nucleus.
Predicted reactivity: Mouse (89%), Rat (93%), Pig (95%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SRC2 (steroid receptor coactivator 2), also known as nuclear receptor coactivator2, NCOA2 or TIF2, aids in the function of nuclear hormone receptors. Nuclear hormone receptors are conditional transcription factors that play important roles in various aspects of cell growth, development, and homeostasis by controlling expression of specific genes. Members of the nuclear hormone receptor superfamily, which includes the 5 steroid receptors and class II nuclear receptors, are structurally characterized by 3 distinct domains: an N-terminal transcriptional activation domain, a central DNA-binding domain, and a C-terminal hormone-binding domain. Before the binding of hormone, steroid receptors, which are sometimes called class I of the nuclear hormone receptor family, remain inactive in a complex with heat-shock protein-90 and other stress family proteins. Binding of hormone induces critical conformational changes in steroid receptors that cause them to dissociate from the inhibitory complex, bind as homodimers to specific DNA enhancer elements associated with target genes, and modulate that gene's transcription. After binding to enhancer elements, transcription factors require transcriptional coactivator proteins to mediate their stimulation of transcription initiation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHe75; c-fgr; c-src2; Class E basic helix-loop-helix protein 75; FLJ43153; glucocorticoid receptor-interacting protein-1; hTIF2; MGC75096; NCoA-2; Nuclear receptor coactivator 2; p160 steroid receptor coactivator 2; p55c-fgr; p58c-fgr; TIF 2; Transcriptional intermediary factor 2
Gene Aliases: BHLHE75; GRIP1; KAT13C; NCoA-2; NCOA2; SRC2; TIF2
UniProt ID: (Human) Q15596
Entrez Gene ID: (Human) 10499
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding
chromatin binding
transcription coactivator activity
histone acetyltransferase activity
protein binding
ligand-dependent nuclear receptor binding
PDZ domain binding
estrogen receptor binding
ligand-dependent nuclear receptor transcription coactivator activity
thyroid hormone receptor coactivator activity
protein complex binding
progesterone receptor binding
nuclear hormone receptor binding
glucocorticoid receptor binding
retinoid X receptor binding
thyroid hormone receptor binding
protein dimerization activity
DNA polymerase binding
histone modifying enzyme
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
male gonad development
regulation of glucose metabolic process
bile acid and bile salt transport
histone acetylation
cerebellum development
intracellular receptor signaling pathway
cellular response to hormone stimulus
circadian regulation of gene expression
cellular lipid metabolic process
locomotor rhythm
positive regulation of female receptivity
positive regulation of transcription from RNA polymerase II promoter
cellular response to Thyroglobulin triiodothyronine
positive regulation of receptor activity
positive regulation of glucocorticoid receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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