|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues G(3) L G E N L D P L A S D S(15) C of human AIB1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 7 publications below|
PA1-845 detects amplified in breast cancer 1 (AIB1) from human tissues and cells.
PA1-845 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~155 kDa protein representing AIB1 from T47D cell lysate.
The PA1-845 immunogen is a synthetic peptide corresponding to residues G(3) L G E N L D P L A S D S(15) C of human AIB1.
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Amplified in breast cancer-1 (AIB-1), is a member of a growing family of transcriptional coactivator proteins which includes steroid receptor coactivator-1 (SRC-1) and glucocorticoid receptor interacting protein-1 (GRIP1).
AIB1/RAC3 has been shown to interact and stimulate transcriptional activity of the retinoic acid, retinoid X, vitamin D, mineralocorticoid, glucocorticoid, thyroid hormone, androgen, progesterone, and peroxisome proliferator activated receptors. AIB1/RAC3 also interacts with the transcriptional integrators CREB binding protein (CBP) and p300.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Uterine endoplasmic reticulum stress-unfolded protein response regulation of gestational length is caspase-3 and -7-dependent.
PA1-845 was used in western blot to analyze the regulation of gestational length in caspase-3 and -7 dependent involving uterine endoplasmic reticulum stress-unfolded protein response
|Kyathanahalli C,Organ K,Moreci RS,Anamthathmakula P,Hassan SS,Caritis SN,Jeyasuria P,Condon JC||Proceedings of the National Academy of Sciences of the United States of America (112:14090)||2015|
Cross-species withdrawal of MCL1 facilitates postpartum uterine involution in both the mouse and baboon.
PA1-845 was used in western blot to investigate the signaling events in pregnant and postnatal uteri of mice and baboons
|Kyathanahalli C,Marks J,Nye K,Lao B,Albrecht ED,Aberdeen GW,Nathanielsz PW,Jeyasuria P,Condon JC||Endocrinology (154:4873)||2013|
Uterine endoplasmic reticulum stress and its unfolded protein response may regulate caspase 3 activation in the pregnant mouse uterus.
PA1-845 was used in western blot to study the regulation of caspase-3 activation in the uterus of pregnant mice by ER stress and unfolded protein responses
|Suresh A,Subedi K,Kyathanahalli C,Jeyasuria P,Condon JC||PloS one (8:null)||2013|
Antiapoptotic signaling via MCL1 confers resistance to caspase-3-mediated apoptotic cell death in the pregnant human uterine myocyte.
PA1-845 was used in western blot to study the effect of MCL1 expression on caspase-3 mediated apoptosis of human uterine myocytes
|Stephenson-Famy A,Marks J,Suresh A,Caritis SN,Simhan H,Jeyasuria P,Condon JC||Molecular endocrinology (Baltimore, Md.) (26:320)||2012|
Electromagnetic fields alter the expression of estrogen receptor cofactors in breast cancer cells.
PA1-845 was used in western blot to investigate the effect of electromagnetic fields on expression of estrogen receptor cofactors in MCF-7 cells
|Girgert R,Gründker C,Emons G,Hanf V||Bioelectromagnetics (29:169)||2008|
Development and therapeutic options for the treatment of raloxifene-stimulated breast cancer in athymic mice.
PA1-845 was used in western blot to investigate the role of HER2/neu during the development of raloxifene-resistant breast cancer.
|O'Regan RM,Osipo C,Ariazi E,Lee ES,Meeke K,Morris C,Bertucci A,Sarker MA,Grigg R,Jordan VC||Clinical cancer research : an official journal of the American Association for Cancer Research (12:2255)||2006|
Factor recruitment and TIF2/GRIP1 corepressor activity at a collagenase-3 response element that mediates regulation by phorbol esters and hormones.
PA1-845 was used in western blot to investigate the role of TIF2/GRIP1 and its associated receptor on specific transcriptional regulation
|Rogatsky I,Zarember KA,Yamamoto KR||The EMBO journal (20:6071)||2001|