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SRC3 Polyclonal Antibody

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SRC3 Antibody in Western Blot (WB) — Western blot of human AIB1 in T47D cell lysate using Product # PA1-845.

Product Details

PA1-845

Applications	Tested Dilution	Publications
Western Blot (WB)	2 µg/mL	View 11 publications 11 publications

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues G(3) L G E N L D P L A S D S(15) C of human AIB1.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2151061

Product Specific Information

PA1-845 detects amplified in breast cancer 1 (AIB1) from human tissues and cells.

PA1-845 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~155 kDa protein representing AIB1 from T47D cell lysate.

The PA1-845 immunogen is a synthetic peptide corresponding to residues G(3) L G E N L D P L A S D S(15) C of human AIB1.

PA1-845 can be used with blocking peptide PEP-049.

Target Information

AIB1-AC3 recognizes AIB1, a member of the SRC-1 family of nuclear receptor coactivators with an apparent molecular weight of 160kDa. The AIB1 gene has been mapped to region 20q12 and has been shown to be amplified in breast tumors and pancreatic cancer cell lines. AIB1 interacts in a ligand-dependent fashion with the estrogen receptor to enhance estrogen-dependent transcriptional gene activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Estrogen receptor alpha isoform ERdelta7 in myometrium modulates uterine quiescence during pregnancy.

EBioMedicine

Anamthathmakula P,Kyathanahalli C,Ingles J,Hassan SS,Condon JC,Jeyasuria P

PA1-845 was used in Western Blotting to identify a role of oestrogen receptor Δ7 as a modulator of myometrial quiescence and define the mechanism of ER Δ7 generation.

Tue Jan 01 00:00:00 EST 2019

Preconditioning the uterine unfolded protein response maintains non-apoptotic Caspase 3-dependent quiescence during pregnancy.

Cell death & disease

Ingles J,Simpson A,Kyathanahalli C,Anamthathmakula P,Hassan S,Jeyasuria P,Condon JC

Published figure using SRC3 polyclonal antibody (Product # PA1-845) in Western Blot

Mon Sep 17 00:00:00 EDT 2018

The Unfolded Protein Response Regulates Uterine Myocyte Antioxidant Responsiveness During Pregnancy.

Biology of reproduction

Ramnarayanan S,Kyathanahalli C,Ingles J,Park-York M,Jeyasuria P,Condon JC

Published figure using SRC3 polyclonal antibody (Product # PA1-845) in Western Blot

Thu Dec 01 00:00:00 EST 2016

Uterine endoplasmic reticulum stress-unfolded protein response regulation of gestational length is caspase-3 and -7-dependent.

Proceedings of the National Academy of Sciences of the United States of America

Kyathanahalli C,Organ K,Moreci RS,Anamthathmakula P,Hassan SS,Caritis SN,Jeyasuria P,Condon JC

PA1-845 was used in western blot to analyze the regulation of gestational length in caspase-3 and -7 dependent involving uterine endoplasmic reticulum stress-unfolded protein response

Tue Nov 10 00:00:00 EST 2015

Uterine endoplasmic reticulum stress and its unfolded protein response may regulate caspase 3 activation in the pregnant mouse uterus.

PloS one

Suresh A,Subedi K,Kyathanahalli C,Jeyasuria P,Condon JC

PA1-845 was used in western blot to study the regulation of caspase-3 activation in the uterus of pregnant mice by ER stress and unfolded protein responses

Tue Sep 30 00:00:00 EDT 2014

Cross-species withdrawal of MCL1 facilitates postpartum uterine involution in both the mouse and baboon.

Endocrinology

Kyathanahalli C,Marks J,Nye K,Lao B,Albrecht ED,Aberdeen GW,Nathanielsz PW,Jeyasuria P,Condon JC

PA1-845 was used in western blot to investigate the signaling events in pregnant and postnatal uteri of mice and baboons

Sun Dec 01 00:00:00 EST 2013

Research resource: identification of novel coregulators specific for thyroid hormone receptor-β2.

Molecular endocrinology (Baltimore, Md.)

Hahm JB,Privalsky ML

PA1-845 was used in Western Blotting to identify a series of previously unrecognized proteins that selectively interact with thyroid hormone receptorΒ2, compared with the more widely expressed TRΒ1.

Wed May 01 00:00:00 EDT 2013

Antiapoptotic signaling via MCL1 confers resistance to caspase-3-mediated apoptotic cell death in the pregnant human uterine myocyte.

Molecular endocrinology (Baltimore, Md.)

Stephenson-Famy A,Marks J,Suresh A,Caritis SN,Simhan H,Jeyasuria P,Condon JC

PA1-845 was used in western blot to study the effect of MCL1 expression on caspase-3 mediated apoptosis of human uterine myocytes

Wed Feb 01 00:00:00 EST 2012

Electromagnetic fields alter the expression of estrogen receptor cofactors in breast cancer cells.

Bioelectromagnetics

Girgert R,Gründker C,Emons G,Hanf V

PA1-845 was used in western blot to investigate the effect of electromagnetic fields on expression of estrogen receptor cofactors in MCF-7 cells

Tue Apr 01 00:00:00 EDT 2008

Development and therapeutic options for the treatment of raloxifene-stimulated breast cancer in athymic mice.

Clinical cancer research : an official journal of the American Association for Cancer Research

O'Regan RM,Osipo C,Ariazi E,Lee ES,Meeke K,Morris C,Bertucci A,Sarker MA,Grigg R,Jordan VC

PA1-845 was used in western blot to investigate the role of HER2/neu during the development of raloxifene-resistant breast cancer.

Sat Apr 01 00:00:00 EST 2006

Factor recruitment and TIF2/GRIP1 corepressor activity at a collagenase-3 response element that mediates regulation by phorbol esters and hormones.

The EMBO journal

Rogatsky I,Zarember KA,Yamamoto KR

PA1-845 was used in western blot to investigate the role of TIF2/GRIP1 and its associated receptor on specific transcriptional regulation

Thu Nov 01 00:00:00 EST 2001

Bioinformatics

Protein Aliases: ACTR; AIB-1; Amplified in breast cancer 1 protein; bHLHe42; CBP-interacting protein; Class E basic helix-loop-helix protein 42; MGC141848; NCoA-3; Nuclear receptor coactivator 3; OTTHUMP00000031716; pCIP; RAC-3; Receptor-associated coactivator 3; RP5-1049G16.3; SRC-3; Steroid receptor coactivator protein 3; Thyroid hormone receptor activator molecule 1; TRAM-1

Gene Aliases: ACTR; AIB-1; AIB1; BHLHE42; CAGH16; CTG26; KAT13B; NCOA3; pCIP; RAC3; SRC-3; SRC3; TNRC14; TNRC16; TRAM-1; TRAM1

UniProt ID: (Human) Q9Y6Q9

Entrez Gene ID: (Human) 8202

Molecular Function: histone modifying enzyme

Function(s)

transcription, DNA-templated positive regulation of gene expression histone acetylation androgen receptor signaling pathway intracellular receptor signaling pathway receptor transactivation positive regulation of keratinocyte differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cellular response to estradiol stimulus regulation of RNA biosynthetic process

Process(es)

transcription coactivator activity histone acetyltransferase activity protein binding ligand-dependent nuclear receptor binding ligand-dependent nuclear receptor transcription coactivator activity nuclear hormone receptor binding thyroid hormone receptor binding protein dimerization activity protein N-terminus binding androgen receptor binding

SRC3 Polyclonal Antibody

Product Details

PA1-845

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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