Immunohistochemical analysis of SRCAP in paraffin-embedded human skin following steamed antigen retrieval with a citrate buffer, pH 6, using a SRCAP polyclonal antibody (Product# PA5-37921) at a dilution of 5ug/ml. Detection was performed using an AP-conjugated secondary antibody.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-QEGNDAEAQRREIE, from the internal region near N-terminus of SRCAP (aa309-322)|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse, rat, dog and pig based on sequence homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.