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SREBF1 Polyclonal Antibody

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SREBF1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 14088-1-AP (SREBF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SREBF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 14088-1-AP (SREBF1 Antibody) at dilution of 1:25 (under 40x lens).

SREBF1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 14088-1-AP (SREBF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SREBF1 Antibody in Immunoprecipitation (IP) — IP result of anti-SREBF1 (IP:14088-1-AP, 4ug; Detection:14088-1-AP 1:600) with L02 cells lysate 1500ug.

Product Details

14088-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 31 publications 31 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 3 publications 3 publications

Product Specifications
Species Reactivity	Human
Published species	Bovine, Chicken, Goat, Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SREBF1 Fusion Protein Ag5219 (1-332 aa encoded by BC063281) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.54 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody can recognize the 125 kDa precursor form and the 68 kDa mature form of human SREBF1.

Immunogen sequence: MDEPPFSEA ALEQALGEPC DLDAALLTDI EGEVGAGRGR ANGLDAPRAG ADRGAMDCTF EDMLQLINNQ DSDFPGLFDP PYAGSGAGGT DPASPDTSSP GSLSPPPATL SSSLEAFLSG PQAAPSPLSP PQPAPTPLKM YPSMPAFSPG PGIKEESVPL SILQTPTPQP LPGALLPQSF PAPAPPQFSS TPVLGYPSPP GGFSTGSPPG NTQQPLPGLP LASPPGVPPV SLHTQVQSVV PQQLLTVTAA PTAAPVTTTV TSQIQQVPVL LQPHFIKADS LLLTAMKTDG ATVKAAGLSP LVSGTTVQTG PLPTLVSGGT ILATVPLVVD AEK (1-332 aa encoded by BC063281)

Target Information

Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SREBP1, targeted by miR-18a-5p, modulates epithelial-mesenchymal transition in breast cancer via forming a co-repressor complex with Snail and HDAC1/2.

Cell death and differentiation

Zhang N,Zhang H,Liu Y,Su P,Zhang J,Wang X,Sun M,Chen B,Zhao W,Wang L,Wang H,Moran MS,Haffty BG,Yang Q

Wed May 01 00:00:00 EDT 2019

SREBP1, targeted by miR-18a-5p, modulates epithelial-mesenchymal transition in breast cancer via forming a co-repressor complex with Snail and HDAC1/2.

Cell death and differentiation

Zhang N,Zhang H,Liu Y,Su P,Zhang J,Wang X,Sun M,Chen B,Zhao W,Wang L,Wang H,Moran MS,Haffty BG,Yang Q

Wed May 01 00:00:00 EDT 2019

Chronic treatment with the modified Longdan Xiegan Tang attenuates olanzapine-induced fatty liver in rats by regulating hepatic de novo lipogenesis and fatty acid beta-oxidation-associated gene expression mediated by SREBP-1c, PPAR-alpha and AMPK-alpha.

Journal of ethnopharmacology

Ren L,Sun D,Zhou X,Yang Y,Huang X,Li Y,Wang C,Li Y

Mon Mar 25 00:00:00 EDT 2019

Magnolol Alleviates Inflammatory Responses and Lipid Accumulation by AMP-Activated Protein Kinase-Dependent Peroxisome Proliferator-Activated Receptor α Activation.

Frontiers in immunology

Tian Y,Feng H,Han L,Wu L,Lv H,Shen B,Li Z,Zhang Q,Liu G

Thu Mar 07 00:00:00 EST 2019

Asiatic acid from Potentilla chinensis alleviates non-alcoholic fatty liver by regulating endoplasmic reticulum stress and lipid metabolism.

International immunopharmacology

Wang D,Lao L,Pang X,Qiao Q,Pang L,Feng Z,Bai F,Sun X,Lin X,Wei J

Sat Dec 01 00:00:00 EST 2018

NR4A1 retards adipocyte differentiation or maturation via enhancing GATA2 and p53 expression.

Journal of cellular and molecular medicine

Qin DD,Yang YF,Pu ZQ,Liu D,Yu C,Gao P,Chen JC,Zong C,Zhang YC,Li X,Wang XD,Liu YT

Mon Oct 01 00:00:00 EDT 2018

Downregulation of microRNA-1 attenuates glucose-induced apoptosis by regulating the liver X receptor α in cardiomyocytes.

Experimental and therapeutic medicine

Cheng Y,Zhao W,Zhang X,Sun L,Yang H,Wang Y,Cao Y,Chu Y,Liu G

Sat Sep 01 00:00:00 EDT 2018

Full-length amyloid precursor protein regulates lipoprotein metabolism and amyloid-β clearance in human astrocytes.

The Journal of biological chemistry

Fong LK,Yang MM,Dos Santos Chaves R,Reyna SM,Langness VF,Woodruff G,Roberts EA,Young JE,Goldstein LSB

Fri Jul 20 00:00:00 EDT 2018

MicroRNA-33b-5p is overexpressed and inhibits GLUT4 by targeting HMGA2 in polycystic ovarian syndrome: An in vivo and in vitro study.

Oncology reports

Yang Y,Jiang H,Xiao L,Yang X

Fri Jun 01 00:00:00 EDT 2018

Mitochondrial ATAD3A regulates milk biosynthesis and proliferation of mammary epithelial cells from dairy cow via the mTOR pathway.

Cell biology international

Chen D,Yuan X,Liu L,Zhang M,Qu B,Zhen Z,Gao X

Tue May 01 00:00:00 EDT 2018

Chronic dexamethasone exposure markedly decreased the hepatic triglyceride accumulation in growing goats.

General and comparative endocrinology

Chen Q,Niu L,Hua C,Geng Y,Cai L,Tao S,Ni Y,Zhao R

Sun Apr 01 00:00:00 EDT 2018

Ophiopogonin D alleviates high-fat diet-induced metabolic syndrome and changes the structure of gut microbiota in mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Chen S,Li X,Liu L,Liu C,Han X

Thu Mar 01 00:00:00 EST 2018

Sulforaphane Improves Abnormal Lipid Metabolism via Both ERS-Dependent XBP1/ACC &SCD1 and ERS-Independent SREBP/FAS Pathways.

Molecular nutrition & food research

Tian S,Li B,Lei P,Yang X,Zhang X,Bao Y,Shan Y

Thu Mar 01 00:00:00 EST 2018

Sulforaphane Improves Abnormal Lipid Metabolism via Both ERS-Dependent XBP1/ACC &SCD1 and ERS-Independent SREBP/FAS Pathways.

Molecular nutrition & food research

Tian S,Li B,Lei P,Yang X,Zhang X,Bao Y,Shan Y

Thu Mar 01 00:00:00 EST 2018

Annexin A2 positively regulates milk synthesis and proliferation of bovine mammary epithelial cells through the mTOR signaling pathway.

Journal of cellular physiology

Zhang M,Chen D,Zhen Z,Ao J,Yuan X,Gao X

Thu Mar 01 00:00:00 EST 2018

Effects of calycosin against high-fat diet-induced nonalcoholic fatty liver disease in mice.

Journal of gastroenterology and hepatology

Duan X,Meng Q,Wang C,Liu Z,Sun H,Huo X,Sun P,Ma X,Peng J,Liu K

Thu Feb 01 00:00:00 EST 2018

DEAD-box helicase 6 (DDX6) is a new negative regulator for milk synthesis and proliferation of bovine mammary epithelial cells.

In vitro cellular & developmental biology. Animal

Zhen Z,Zhang M,Yuan X,Qu B,Yu Y,Gao X,Qiu Y

Mon Jan 01 00:00:00 EST 2018

Baicalin attenuates non-alcoholic steatohepatitis by suppressing key regulators of lipid metabolism, inflammation and fibrosis in mice.

Life sciences

Zhang J,Zhang H,Deng X,Zhang N,Liu B,Xin S,Li G,Xu K

Mon Jan 01 00:00:00 EST 2018

Formaldehyde alters triglyceride synthesis and very low-density lipoprotein secretion in a time-dependent manner.

Environmental toxicology and pharmacology

Bai J,Wang P,Liu Y,Zhang Y,Li Y,He Z,Hou L,Liang R

Fri Dec 01 00:00:00 EST 2017

Twinfilin 1 enhances milk bio-synthesis and proliferation of bovine mammary epithelial cells via the mTOR signaling pathway.

Biochemical and biophysical research communications

Li L,Liu L,Qu B,Li X,Gao X,Zhang M

Sat Oct 21 00:00:00 EDT 2017

Dihydrocapsaicin Attenuates Plaque Formation through a PPARγ/LXRα Pathway in apoE(-/-) Mice Fed a High-Fat/High-Cholesterol Diet.

PloS one

Hu YW,Ma X,Huang JL,Mao XR,Yang JY,Zhao JY,Li SF,Qiu YR,Yang J,Zheng L,Wang Q

Thu Oct 12 00:00:00 EDT 2017

Prenatal betaine exposure alleviates corticosterone-induced inhibition of CYP27A1 expression in the liver of juvenile chickens associated with its promoter DNA methylation.

General and comparative endocrinology

Hu Y,Sun Q,Zong Y,Liu J,Idriss AA,Omer NA,Zhao R

Mon May 15 00:00:00 EDT 2017

B7-H3 regulates lipid metabolism of lung cancer through SREBP1-mediated expression of FASN.

Biochemical and biophysical research communications

Luo D,Xiao H,Dong J,Li Y,Feng G,Cui M,Fan S

Sun Jan 22 00:00:00 EST 2017

Alisol B 23-acetate protects against non-alcoholic steatohepatitis in mice via farnesoid X receptor activation.

Acta pharmacologica Sinica

Meng Q,Duan XP,Wang CY,Liu ZH,Sun PY,Huo XK,Sun HJ,Peng JY,Liu KX

Sun Jan 01 00:00:00 EST 2017

Medium-chain fatty acid reduces lipid accumulation by regulating expression of lipid-sensing genes in human liver cells with steatosis.

International journal of food sciences and nutrition

Wang B,Fu J,Li L,Gong D,Wen X,Yu P,Zeng Z

Tue Dec 13 00:00:00 EST 2016

Protective effects of glycyrrhizic acid from edible botanical glycyrrhiza glabra against non-alcoholic steatohepatitis in mice.

Food & function

Wang C,Duan X,Sun X,Liu Z,Sun P,Yang X,Sun H,Liu K,Meng Q

Wed Sep 14 00:00:00 EDT 2016

VNN1 promotes atherosclerosis progression in apoE-/- mice fed a high-fat/high-cholesterol diet.

Journal of lipid research

Hu YW,Wu SG,Zhao JJ,Ma X,Lu JB,Xiu JC,Zhang Y,Huang C,Qiu YR,Sha YH,Gao JJ,Wang YC,Li SF,Zhao JY,Zheng L,Wang Q

Mon Aug 01 00:00:00 EDT 2016

MicroRNA-24 promotes 3T3-L1 adipocyte differentiation by directly targeting the MAPK7 signaling.

Biochemical and biophysical research communications

Jin M,Wu Y,Wang J,Chen J,Huang Y,Rao J,Feng C

Fri May 20 00:00:00 EDT 2016

LXRalpha gene downregulation by lentiviral-based RNA interference enhances liver function after fatty liver transplantation in rats.

Hepatobiliary & pancreatic diseases international : HBPD INT

Zhao YP,Li L,Ma JP,Chen G,Bai JH

Sat Aug 01 00:00:00 EDT 2015

ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer.

Cell research

Ji S,Qin Y,Shi S,Liu X,Hu H,Zhou H,Gao J,Zhang B,Xu W,Liu J,Liang D,Liu L,Liu C,Long J,Zhou H,Chiao PJ,Xu J,Ni Q,Gao D,Yu X

Fri May 01 00:00:00 EDT 2015

Supplementation of chitosan alleviates high-fat diet-enhanced lipogenesis in rats via adenosine monophosphate (AMP)-activated protein kinase activation and inhibition of lipogenesis-associated genes.

Journal of agricultural and food chemistry

Chiu CY,Chan IL,Yang TH,Liu SH,Chiang MT

Wed Mar 25 00:00:00 EDT 2015

Long noncoding RNA HULC modulates abnormal lipid metabolism in hepatoma cells through an miR-9-mediated RXRA signaling pathway.

Cancer research

Cui M,Xiao Z,Wang Y,Zheng M,Song T,Cai X,Sun B,Ye L,Zhang X

Sun Mar 01 00:00:00 EST 2015

RP5-833A20.1/miR-382-5p/NFIA-dependent signal transduction pathway contributes to the regulation of cholesterol homeostasis and inflammatory reaction.

Arteriosclerosis, thrombosis, and vascular biology

Hu YW,Zhao JY,Li SF,Huang JL,Qiu YR,Ma X,Wu SG,Chen ZP,Hu YR,Yang JY,Wang YC,Gao JJ,Sha YH,Zheng L,Wang Q

Thu Jan 01 00:00:00 EST 2015

RP5-833A20.1/miR-382-5p/NFIA-dependent signal transduction pathway contributes to the regulation of cholesterol homeostasis and inflammatory reaction.

Arteriosclerosis, thrombosis, and vascular biology

Hu YW,Zhao JY,Li SF,Huang JL,Qiu YR,Ma X,Wu SG,Chen ZP,Hu YR,Yang JY,Wang YC,Gao JJ,Sha YH,Zheng L,Wang Q

Thu Jan 01 00:00:00 EST 2015

A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis.

Journal of lipid research

Hu YW,Yang JY,Ma X,Chen ZP,Hu YR,Zhao JY,Li SF,Qiu YR,Lu JB,Wang YC,Gao JJ,Sha YH,Zheng L,Wang Q

Tue Apr 01 00:00:00 EDT 2014

Pleiotropic effects of cavin-1 deficiency on lipid metabolism.

The Journal of biological chemistry

Ding SY,Lee MJ,Summer R,Liu L,Fried SK,Pilch PF

Fri Mar 21 00:00:00 EDT 2014

PloS one

Zhou T,Li S,Zhong W,Vihervaara T,Béaslas O,Perttilä J,Luo W,Jiang Y,Lehto M,Olkkonen VM,Yan D

Mon Nov 07 00:00:00 EST 2011

Bioinformatics

Protein Aliases: bHLHd1; Class D basic helix-loop-helix protein 1; nt-SREBP1; Processed sterol regulatory element-binding protein 1; SREBP-1; Sterol regulatory element-binding protein 1; Sterol regulatory element-binding transcription factor 1; Transcription factor SREBF1

Gene Aliases: BHLHD1; SREBF1; SREBP-1c; SREBP1; SREBP1a

UniProt ID: (Human) P36956

Entrez Gene ID: (Human) 6720

Function(s)

negative regulation of transcription from RNA polymerase II promoter regulation of heart rate by chemical signal regulation of transcription from RNA polymerase II promoter lipid metabolic process aging circadian rhythm cholesterol metabolic process insulin receptor signaling pathway lipid biosynthetic process cellular response to starvation response to glucose positive regulation of triglyceride biosynthetic process regulation of fatty acid metabolic process lung development positive regulation of histone deacetylation regulation of protein stability response to food response to retinoic acid response to progesterone response to glucagon response to drug mRNA transcription from RNA polymerase II promoter fat cell differentiation positive regulation of cholesterol biosynthetic process positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion response to cAMP cellular response to fatty acid regulation of mitophagy regulation of protein targeting to mitochondrion

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding protein kinase binding protein complex binding sterol response element binding protein dimerization activity

SREBF1 Polyclonal Antibody

Product Details

14088-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Immunohistochemistry (IHC)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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