PA1-337 detects SREBP 1 in mouse and rat samples.
PA1-337 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~68 and 120 kDa protein representing SREBP 1 in mouse and rat liver samples as well as rat kidney samples. A predominent band at ~68 kDa (active cleaved site) is seen and a band at ~120 kDa (inactive precursor) may not be seen or it may be diminished.
The PA1-337 immunogen is a synthetic peptide corresponding to residues M(32) L Q L I N N Q D S D F P G L F(47) of mouse SREBP 1. This sequence is identical in human and rat. PA1-337 immunizing peptide (Cat. # PEP-274) is available for use in neutralization and control experiments.
Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2.
SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment.
In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ADD1; Adipocyte determination- and differentiation-dependent factor 1; bHLHd1; Class D basic helix-loop-helix protein 1; nt-SREBP1; Processed sterol regulatory element-binding protein 1; SREBP-1; Sterol regulatory element binding protein 1; Sterol regulatory element-binding protein 1; Sterol regulatory element-binding transcription factor 1
Gene Aliases: ADD-1; ADD1; BHLHD1; D630008H06; SREBF1; SREBP-1; SREBP-1a; SREBP-1c; SREBP1; SREBP1a; SREBP1c