Invitrogen

SREBP2 Polyclonal Antibody

2 Published Figures

8 References

View all (19) SREBP2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (2)

SREBP2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

SREBP2 Antibody (PA1-338) in ICC/IF

Immunofluorescent analysis of SREBP2 (green) showing staining in the cytoplasm of PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a SREBP2 polyclonal antibody (Product # PA1-338) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin... View More

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Product Details

PA1-338

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 7 publications 7 publications

Immunocytochemistry (ICC/IF)

1:20-1:200

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues A(843) I S W L Q G D D A A V R S H(857) of rat SREBP 2.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2194237

Product Specific Information

PA1-338 detects SREBP 2 in human, mouse and rat samples.

The PA1-338 immunogen is a synthetic peptide corresponding to residues A(843) I S W L Q G D D A A V R S H(857) of rat SREBP 2. This sequence is identical in human and differs by 1 a.a. in mouse. PA1-338 immunizing peptide (Cat. # PEP-275) is available for use in neutralization and control experiments.

Target Information

This gene encodes a ubiquitously expressed transcription factor that controls cholesterol homeostasis by stimulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TFEB inhibition induces melanoma shut-down by blocking the cell cycle and rewiring metabolism.

Cell death & disease

Ariano C,Costanza F,Akman M,Riganti C,Corà D,Casanova E,Astanina E,Comunanza V,Bussolino F,Doronzo G

PA1-338 was used in Western Blot to suggest that the oncogenic transcription factor EB (TFEB), a key regulator of lysosomal biogenesis and function, controls melanoma tumour growth through a transcriptional programme targeting ERK1/2 activity and glucose, glutamine and cholesterol metabolism.

Tue May 09 00:00:00 UTC 2023

N¹-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Nature communications

Wang Y,Wang J,Li X,Xiong X,Wang J,Zhou Z,Zhu X,Gu Y,Dominissini D,He L,Tian Y,Yi C,Fan Z

Published figure using SREBP2 polyclonal antibody (Product # PA1-338) in Western Blot

Tue Nov 02 00:00:00 UTC 2021

N¹-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Nature communications

Wang Y,Wang J,Li X,Xiong X,Wang J,Zhou Z,Zhu X,Gu Y,Dominissini D,He L,Tian Y,Yi C,Fan Z

Published figure using SREBP2 polyclonal antibody (Product # PA1-338) in Western Blot

Tue Nov 02 00:00:00 UTC 2021

The cholesterol metabolite 25-hydroxycholesterol restrains the transcriptional regulator SREBP2 and limits intestinal IgA plasma cell differentiation.

Immunity

Trindade BC,Ceglia S,Berthelette A,Raso F,Howley K,Muppidi JR,Reboldi A

PA1-338 was used in Immunocytochemistry, Western Blot, Flow Cytometry to examine how the cholesterol metabolite 25-hydroxycholesterol (25-HC) impacts the intestinal B cell response.

Tue Oct 12 00:00:00 UTC 2021

Autophagy regulates fatty acid availability for oxidative phosphorylation through mitochondria-endoplasmic reticulum contact sites.

Nature communications

Bosc C,Broin N,Fanjul M,Saland E,Farge T,Courdy C,Batut A,Masoud R,Larrue C,Skuli S,Espagnolle N,Pagès JC,Carrier A,Bost F,Bertrand-Michel J,Tamburini J,Récher C,Bertoli S,Mansat-De Mas V,Manenti S,Sarry JE,Joffre C

PA1-338 was used in Western Blot to establish that mitochondria, through the regulation of MERCs, controls autophagy that, in turn finely tunes lipid degradation to fuel OxPHOS supporting proliferation and growth in leukemia.

Thu Aug 13 00:00:00 UTC 2020

p53 Represses the Mevalonate Pathway to Mediate Tumor Suppression.

Cell

Moon SH,Huang CH,Houlihan SL,Regunath K,Freed-Pastor WA,Morris JP,Tschaharganeh DF,Kastenhuber ER,Barsotti AM,Culp-Hill R,Xue W,Ho YJ,Baslan T,Li X,Mayle A,de Stanchina E,Zender L,Tong DR,D'Alessandro A,Lowe SW,Prives C

PA1-338 was used in Western Blotting to demonstrate that repression of the mevalonate pathway is a crucial component of p53-mediated liver tumor suppression and report on the mechanisms involved in this.

Thu Jan 24 00:00:00 UTC 2019

Absence of gravin-mediated signaling inhibits development of high-fat diet-induced hyperlipidemia and atherosclerosis.

American journal of physiology. Heart and circulatory physiology

Fan Q,Yin X,Rababa'h A,Diaz Diaz A,Wijaya CS,Singh S,Suryavanshi SV,Vo HH,Saeed M,Zhang Y,McConnell BK

PA1-338 was used in Western Blotting to investigate the role of gravin in the development of high-fat diet (HFD)-induced atherosclerosis and hyperlipidemia.

Tue Oct 01 00:00:00 UTC 2019

Pleiotropic Role of p53 in Injury and Liver Regeneration after Acetaminophen Overdose.

The American journal of pathology

Borude P,Bhushan B,Gunewardena S,Akakpo J,Jaeschke H,Apte U

PA1-338 was used in Western Blotting to investigate the role of p53 in liver injury and regeneration after acetaminophen overdose.

Fri Jun 01 00:00:00 UTC 2018

High copper selectively alters lipid metabolism and cell cycle machinery in the mouse model of Wilson disease.

The Journal of biological chemistry

Huster D,Purnat TD,Burkhead JL,Ralle M,Fiehn O,Stuckert F,Olson NE,Teupser D,Lutsenko S

PA1-338 was used in western blot to investigate the role of SREBP2 during the copper-induced alteration of lipid metabolism and cell cycle machinery.

Fri Mar 16 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: bHLHd2; Class D basic helix-loop-helix protein 2; srebf2; srebf2 {ECO:0000312|EMBL:AAI01903.1}; SREBP-2; sterol regulatory element binding factor 2; sterol regulatory element binding protein 2; Sterol regulatory element-binding protein 2; Sterol regulatory element-binding transcription factor 2

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Gene Aliases: AI608257; BHLHD2; lop13; nuc; SREBF2; Srebf2_retired; SREBP-2; SREBP2; SREBP2gc

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UniProt ID: (Human) Q12772, (Rat) Q3T1I5, (Mouse) Q3U1N2

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Entrez Gene ID: (Human) 6721, (Rat) 300095, (Mouse) 20788

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Function(s)

C-8 sterol isomerase activity RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding protein C-terminus binding protein dimerization activity E-box binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding transcription regulatory region DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated spermatogenesis aging cholesterol metabolic process lipid biosynthetic process response to hormone response to lead ion positive regulation of cholesterol storage response to lipid response to drug positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter response to low-density lipoprotein particle regulation of lipid transport by negative regulation of transcription from RNA polymerase II promoter negative regulation of cholesterol efflux regulation of cholesterol homeostasis lipid metabolic process cellular response to starvation cellular response to laminar fluid shear stress regulation of mitophagy positive regulation of protein targeting to mitochondrion transcription from RNA polymerase II promoter steroid metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SREBP2 Polyclonal Antibody

SREBP2 Antibody (PA1-338) in ICC/IF

Product Details

PA1-338

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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