Invitrogen

SREBP2 Polyclonal Antibody

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SREBP2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of SREBP2 (green) showing staining in the cytoplasm of PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a SREBP2 polyclonal antibody (Product # PA1-338) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

SREBP2 Antibody in Western Blot (WB) — Fig. 4 PPARdelta promotes liver CSC self-renewal via enhancing cholesterol synthesis. a Heatmap analysis of signature molecules of steroid metabolites under positive ion mode in PPARdelta depleted versus sgCtrl treated liver CSC cells. b Heatmap analysis of cholesterol metabolism related genes in PPARdelta-depleted and control CSCs. Data were normalized to endogenous 18S rRNA expression and sgCtrl were assigned with a value of 1. c Kaplan-Meier plots of overall survival for SREBF2 in TMA cohort. Median expression value was used as a cut-off. P values for Kaplan-Meier curve were determined using a two-sided log-rank test. d Western blotting analysis of cholesterol metabolism related gene expression in PPARdelta depleted, GSK3787 (20 muM) treated and SREBF2 overexpressed liver CSCs compared with control CSCs. n = 3 biologically independent samples. e Levels of total cholesterol, free cholesterol and cholesterol esterin TRMT6/TRMT61A, PPARdelta, and SREBF2 depleted liver CSCs. Data are means +- SD. n = 6. Exact P values from left to right: 0.037, 0.0061, 0.0070, 0.0032, 0.046. f Filipin III staining of cellular cholesterol distributions in PPARdelta depleted, and co-transfection of SREBF2 in PPARdelta depleted liver CSCs from two HCC patients compared with control CSCs ( n = 5). Scale bar, 10 mum. n = 3 biologically independent samples. g Total cholesterol levels in liver CSCs treated with vehicle (DMSO), PPARdelta antagonist (GSK3787, 20 muM) or agonist (GW501516, 20 muM) for 7

Product Details

PA1-338

Applications	Tested Dilution	Publications
Western Blot (WB)	1:100-1:1,000	View 6 publications 6 publications
Immunocytochemistry (ICC/IF)	1:20-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues A(843) I S W L Q G D D A A V R S H(857) of rat SREBP 2.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2194237

Product Specific Information

PA1-338 detects SREBP 2 in human, mouse and rat samples.

The PA1-338 immunogen is a synthetic peptide corresponding to residues A(843) I S W L Q G D D A A V R S H(857) of rat SREBP 2. This sequence is identical in human and differs by 1 a.a. in mouse. PA1-338 immunizing peptide (Cat. # PEP-275) is available for use in neutralization and control experiments.

Target Information

This gene encodes a ubiquitously expressed transcription factor that controls cholesterol homeostasis by stimulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

N¹-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Nature communications

Wang Y,Wang J,Li X,Xiong X,Wang J,Zhou Z,Zhu X,Gu Y,Dominissini D,He L,Tian Y,Yi C,Fan Z

PA1-338 was used in Western Blot to show that N1-methyladenosine methylation (m1A) in tRNA is remarkably elevated in hepatocellular carcinoma (HCC) patient tumour tissues.

Tue Nov 02 00:00:00 EDT 2021

N¹-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Nature communications

Wang Y,Wang J,Li X,Xiong X,Wang J,Zhou Z,Zhu X,Gu Y,Dominissini D,He L,Tian Y,Yi C,Fan Z

PA1-338 was used in Western Blot to show that N1-methyladenosine methylation (m1A) in tRNA is remarkably elevated in hepatocellular carcinoma (HCC) patient tumour tissues.

Tue Nov 02 00:00:00 EDT 2021

Autophagy regulates fatty acid availability for oxidative phosphorylation through mitochondria-endoplasmic reticulum contact sites.

Nature communications

Bosc C,Broin N,Fanjul M,Saland E,Farge T,Courdy C,Batut A,Masoud R,Larrue C,Skuli S,Espagnolle N,Pagès JC,Carrier A,Bost F,Bertrand-Michel J,Tamburini J,Récher C,Bertoli S,Mansat-De Mas V,Manenti S,Sarry JE,Joffre C

PA1-338 was used in Western Blot to establish that mitochondria, through the regulation of MERCs, controls autophagy that, in turn finely tunes lipid degradation to fuel OxPHOS supporting proliferation and growth in leukemia.

Thu Aug 13 00:00:00 EDT 2020

Absence of gravin-mediated signaling inhibits development of high-fat diet-induced hyperlipidemia and atherosclerosis.

American journal of physiology. Heart and circulatory physiology

Fan Q,Yin X,Rababa'h A,Diaz Diaz A,Wijaya CS,Singh S,Suryavanshi SV,Vo HH,Saeed M,Zhang Y,McConnell BK

PA1-338 was used in Western Blotting to investigate the role of gravin in the development of high-fat diet (HFD)-induced atherosclerosis and hyperlipidemia.

Tue Oct 01 00:00:00 EDT 2019

p53 Represses the Mevalonate Pathway to Mediate Tumor Suppression.

Cell

Moon SH,Huang CH,Houlihan SL,Regunath K,Freed-Pastor WA,Morris JP,Tschaharganeh DF,Kastenhuber ER,Barsotti AM,Culp-Hill R,Xue W,Ho YJ,Baslan T,Li X,Mayle A,de Stanchina E,Zender L,Tong DR,D'Alessandro A,Lowe SW,Prives C

PA1-338 was used in Western Blotting to demonstrate that repression of the mevalonate pathway is a crucial component of p53-mediated liver tumor suppression and report on the mechanisms involved in this.

Thu Jan 24 00:00:00 EST 2019

Pleiotropic Role of p53 in Injury and Liver Regeneration after Acetaminophen Overdose.

The American journal of pathology

Borude P,Bhushan B,Gunewardena S,Akakpo J,Jaeschke H,Apte U

PA1-338 was used in Western Blotting to investigate the role of p53 in liver injury and regeneration after acetaminophen overdose.

Fri Jun 01 00:00:00 EDT 2018

High copper selectively alters lipid metabolism and cell cycle machinery in the mouse model of Wilson disease.

The Journal of biological chemistry

Huster D,Purnat TD,Burkhead JL,Ralle M,Fiehn O,Stuckert F,Olson NE,Teupser D,Lutsenko S

PA1-338 was used in western blot to investigate the role of SREBP2 during the copper-induced alteration of lipid metabolism and cell cycle machinery.

Fri Mar 16 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: bHLHd2; Class D basic helix-loop-helix protein 2; Processed sterol regulatory element-binding protein 2; srebf2; srebf2 {ECO:0000312|EMBL:AAI01903.1}; SREBP-2; sterol regulatory element binding factor 2; sterol regulatory element binding protein 2; Sterol regulatory element-binding protein 2; Sterol regulatory element-binding transcription factor 2; Transcription factor SREBF2

Gene Aliases: AI608257; BHLHD2; lop13; nuc; SREBF2; Srebf2_retired; SREBP-2; SREBP2; SREBP2gc

UniProt ID: (Human) Q12772, (Rat) Q3T1I5, (Mouse) Q3U1N2

Entrez Gene ID: (Human) 6721, (Rat) 300095, (Mouse) 20788

Function(s)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated spermatogenesis aging cholesterol metabolic process lipid biosynthetic process response to hormone response to lead ion positive regulation of cholesterol storage response to lipid response to drug positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter response to low-density lipoprotein particle regulation of lipid transport by negative regulation of transcription from RNA polymerase II promoter negative regulation of cholesterol efflux regulation of cholesterol homeostasis lipid metabolic process cellular response to starvation cellular response to laminar fluid shear stress regulation of mitophagy positive regulation of protein targeting to mitochondrion transcription from RNA polymerase II promoter steroid metabolic process

Process(es)

C-8 sterol isomerase activity RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding protein C-terminus binding protein dimerization activity E-box binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding transcription regulatory region DNA binding

SREBP2 Polyclonal Antibody

Product Details

PA1-338

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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