|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 136 of Human SRP14|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 40% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-27554 targets SRP14 in WB applications and shows reactivity with Human samples.
The PA5-27554 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 136 of Human SRP14.
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A functional link between the co-translational protein translocation pathway and the UPR.
PA5-27554 was used in western blot to elucidate the mechanism by which Ire1alpha cleaves mRNAs at the ER membrane.
|Plumb R,Zhang ZR,Appathurai S,Mariappan M||eLife (4:null)||2015|