Invitrogen

SRP54 Polyclonal Antibody

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SRP54 Antibody in Western Blot (WB) — Western Blot of MCF7, 293T using a Polyclonal SRP54 Antibody (Product # PA5-113697). Positive control (+): MCF7 (N10). Negative control (-): 293T (2T). Antibody concentration: 1 µg/mL.

SRP54 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of HEK293 Whole Cell Lysate using a SRP54 Polyclonal Antibody (Product # PA5-113697). SRP54 was immunoprecipitated from 1 mg HEK293 Whole Cell Lysate with PA5-113697 diluted 1:200. Western blot was performed using PA5-113697 diluted 1:1,000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: SRP54 IP with PA5-113697 in HEK293 Whole Cell Lysate. Lane 3: Input of HEK293 Whole Cell Lysate.

Product Details

PA5-113697

Applications	Tested Dilution	Publications
Western Blot (WB)	0.2-1 µg/mL	-
Immunoprecipitation (IP)	1:200	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide directed towards the middle region of human SRP54
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 2% sucrose
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2884212

Product Specific Information

Immunogen sequence: ENFEIIIVDT SGRHKQEDSL FEEMLQVANA IQPDNIVYVM DASIGQACEA

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Predicted homology: Cow: 100%; Dog: 100%; Goat: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%

Target Information

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.