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SRPK2 Antibody (PA5-110430) in WB
Western blot analysis of SRPK2 in extracts of various cell lines. Samples were incubated with SRPK2 Polyclonal antibody (Product # PA5-110430) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.
Product Details
PA5-110430
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 420-520 of human SRPK2 (NP_872633.1).
Conjugate
Form
Liquid
Concentration
1.06 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2855841
Product Specific Information
Immunogen sequence: NAESDYTYSS SYEQFNGELP NGRHKIPESQ FPEFSTSLFS GSLEPVACGS VLSEGSPLTE QEESSPSHDR SRTVSASSTG DLPKAKTRAA DLLVNPLDPR N
Target Information
SRPK2, along with SRPK1, is most likely the cellular protein kinase mediating HBV core protein phosphorylation during viral infection. It therefore represents an important host cell target for therapeutic intervention in HBV infection. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles .
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H_RG152G17.1a; H_RG152G17.1b; serine kinase SRPK2; serine/arginine-rich protein specific kinase 2; Serine/arginine-rich protein-specific kinase 2; serine/arginine-rich splicing factor kinase 2; serine/threonine-protein kinase SRPK2; SFRS protein kinase 2; SR protein kinase 2; SR-protein-specific kinase 2; SRPK2; SRSF protein kinase 2; WUGSC:H_RG152G17.1a; WW domain binding protein 6
Gene Aliases: AW226533; AW492537; AW547358; mSRPK2; SFRSK2; SRPK2; Wbp6
UniProt ID: (Human) P78362, (Mouse) O54781
Entrez Gene ID: (Human) 6733, (Rat) 296753, (Mouse) 20817
magnesium ion binding
protein serine/threonine kinase activity
protein binding
ATP binding
poly(A) RNA binding
14-3-3 protein binding
protein kinase activity
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
spliceosomal complex assembly
angiogenesis
protein phosphorylation
positive regulation of cell proliferation
RNA splicing
positive regulation of gene expression
cell differentiation
nuclear speck organization
intracellular signal transduction
positive regulation of neuron apoptotic process
positive regulation of viral genome replication
negative regulation of viral genome replication
innate immune response
positive regulation of cell cycle
regulation of mRNA splicing, via spliceosome
mRNA processing
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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