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Invitrogen
SRSF3 Polyclonal Antibody
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FIGURE: 1 / 2
SRSF3 Antibody (PA5-101141) in ICC/IF
Immunocytochemistry analysis of SRSF3 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SRSF3 polyclonal antibody (Product # PA5-101141) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101141
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SRSF3(Accession P84103), corresponding to amino acid residues R131-K164.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total SFRS3.
Target Information
SR proteins appear to be recruited from nuclear "speckles", in which they are concentrated, to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: pre-mRNA splicing factor SRp20; Pre-mRNA-splicing factor SRP20; Protein X16; Serine/arginine-rich splicing factor 3; Splicing factor, arginine/serine-rich 3; splicing factor, arginine/serine-rich 3 (SRp20); splicing factor, arginine/serine-rich, 20-kD
Gene Aliases: AL024116; SFRS3; SRP20; SRSF3; X16
UniProt ID: (Human) P84103, (Mouse) P84104
Entrez Gene ID: (Human) 6428, (Mouse) 20383
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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