Western blot analysis of SS18 using A) 30 µg A549 whole cell lysate (B) 30 µg H1299 whole cell lysate and C) 30 µg HCT116 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a SS18 polyclonal antibody (Product # PA5-35989) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 136 and 270 of SS18|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Appears to function synergistically with RBM14 as a transcriptional coactivator. Isoform 1 and isoform 2 function in nuclear receptor coactivation. Isoform 1 and isoform 2 function in general transcriptional coactivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.