Immunofluorescent analysis of SSBP1 (red) in HeLa cells. HeLa cells were fixed with 4% paraformaldyhyde, permeabilized with 1% Triton X-100 in PBS and blocked with 10% goat serum. Cells were probed with a SSBP1 polyclonal antibody (Product # PA1-27871) at a dilution of 1:1000 followed by a fluorescently-conjugated goat anti-chicken IgY secondary antibody. Data courtesy of the Innovators Program.
|Tested species reactivity||Mouse, Human, Rat|
|Host / Isotype||Chicken / IgY|
|Immunogen||Synthetic peptide corresponding to human Single-stranded DNA-binding protein (mitochondrial)|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-27871 detects SSBP1 from human, mouse, and rat samples.
PA1-27871 may be successfully used in IF and Western blot applications. By Western blot, this antibody has only been tested on fusion protein.
The immunogen is a synthetic peptide corresponding to human single-stranded DNA-binding protein (mitochondrial).
This protein binds preferentially and cooperatively to ss-DNA. Probably involved in mitochondrial DNA replication. SSBP is a housekeeping gene involved in mitochondrial biogenesis (Tiranti et al.. 1995).[supplied by OMIM].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.