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Product Details
620-500
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
0.1-1 µg/mL | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
2-10 µg/mL | - |
Immunocytochemistry (ICC/IF) |
1:200 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
A synthetic peptide derived from the C-terminus of human SSEA-4 protein |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS with proprietary stabilizer |
Contains |
0.01% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Product Specific Information
Positive control: TRA1
Cellular location: Membrane Cytoplasm.
Target Information
SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: SSEA-4; stage-specific embryonic antigen 4
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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