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Invitrogen
SSR3 Polyclonal Antibody
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FIGURE: 1 / 12
SSR3 Antibody (PA5-80072) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of SSR3 in HELA cell using SSR3 Polyclonal Antibody (Product # PA5-80072). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 1 µg/mL. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Product Details
PA5-80072
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SSR3 (8-23aa KQQSEEDLLLQDFSRN).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human HepG2 whole cell, human K562 whole cell, monkey COS-7 whole cell, rat C6 whole cell, mouse RAW2647 whole cell. IHC: human breast cancer tissue, human placenta tissue, human lung cancer tissue, mouse cerebral cortex tissue, mouse hippocampus tissue, rat cerebral cortex tissue, rat hippocampus tissue. ICC/IF: HELA cell.
Target Information
SSR3 (signal sequence receptor, gamma) is also known as TRAPG. By immunoaffinity chromatography, biochemical purification, peptide sequence analysis, and screening of a rat liver cDNA library, Hartmann et al. (1993) obtained a cDNA encoding Ssr3, which they termed Trap-gamma. The deduced 185-amino acid protein spans the membrane 4 times and is expressed as a 20-kD protein. Gross (2012)mapped the SSR3 gene to chromosome 3q25.31 based on an alignment of the SSR3 sequence with the genomic sequence (GRCh37). Hartmann et al. (1993)found that Ssr3 was associated in equal amounts with Ssr1, Ssr2, and Ssr4 in canine pancreatic microsomes.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Signal sequence receptor subunit gamma; signal sequence receptor, gamma (translocon-associated protein gamma); SSR gamma; SSR-gamma; translocon-associated protein gamma; translocon-associated protein gamma subunit; Translocon-associated protein subunit gamma; TRAP-complex gamma subunit; TRAP-gamma; unnamed protein product
Gene Aliases: 0610038P07Rik; AL022999; AU022074; AW553833; SSR3; TRAPG
UniProt ID: (Human) Q9UNL2, (Mouse) Q9DCF9, (Rat) Q08013
Entrez Gene ID: (Human) 6747, (Mouse) 67437, (Rat) 81784
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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