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STAT1 Antibody (710078) in WB
Western blot was performed using Anti-STAT1 Recombinant Rabbit Superclonal™ Antibody (Product # 710078) and a 87 kDa band corresponding to STAT1 was observed in HeLa cells upon IFN gamma treatment. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with IFN-gamma (1000u/mL for 4 Hours) (Lane 2) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The b... View More
Product Details
710078
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
4HCLC
Immunogen
Recombinant protein corresponding to amino acids 134-218 of human STAT1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp686B04100; OTTHUMP00000165046; OTTHUMP00000205845; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; Signal transducer and activator of transcription 1-alpha/beta; STAT; STAT 1; STAT-1; stat1 alpha; transcription factor; Transcription factor ISGF-3 components p91/p84; transcription factor; 91 kDa; unnamed protein product
Gene Aliases: CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT1; STAT91
UniProt ID: (Human) P42224
Entrez Gene ID: (Human) 6772
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription corepressor binding
transcription coactivator binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
tumor necrosis factor receptor binding
protein binding
enzyme binding
CCR5 chemokine receptor binding
histone acetyltransferase binding
histone binding
identical protein binding
protein homodimerization activity
sequence-specific DNA binding
ubiquitin-like protein ligase binding
cadherin binding
protein phosphatase 2A binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
negative regulation of endothelial cell proliferation
positive regulation of mesenchymal cell proliferation
positive regulation of defense response to virus by host
negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
defense response
signal transduction
JAK-STAT cascade
response to nutrient
blood circulation
positive regulation of cell proliferation
response to xenobiotic stimulus
response to mechanical stimulus
negative regulation of angiogenesis
positive regulation of interferon-alpha production
cellular response to insulin stimulus
tumor necrosis factor-mediated signaling pathway
response to cytokine
response to interferon-gamma
response to interferon-beta
cellular response to interferon-beta
interleukin-7-mediated signaling pathway
interleukin-9-mediated signaling pathway
regulation of cell proliferation
response to hydrogen peroxide
regulation of apoptotic process
negative regulation of I-kappaB kinase/NF-kappaB signaling
response to peptide hormone
endothelial cell migration
positive regulation of nitric oxide biosynthetic process
positive regulation of erythrocyte differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JAK-STAT cascade
negative regulation by virus of viral protein levels in host cell
positive regulation of smooth muscle cell proliferation
negative regulation of developmental process
response to cAMP
defense response to virus
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
renal tubule development
interleukin-27-mediated signaling pathway
cellular response to cytokine stimulus
cellular response to interferon-gamma
metanephric mesenchymal cell proliferation involved in metanephros development
metanephric mesenchymal cell differentiation
negative regulation of metanephric nephron tubule epithelial cell differentiation
receptor signaling pathway via STAT
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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