|Western Blot (WB)||1µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues D(830) E V Y V S R P S H F Y T D G P L M P S(849) of human STAT2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
OPA1-03005 detects signal transducer and activator of transcription 2 (STAT2) from human tissues and cells. This antibody is specific for STAT2 and does not cross-react with any other STATs or related protein.
OPA1-03005 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~113 kDa protein representing STAT2 from HeLa cell lysate.
OPA1-03005 immunizing peptide corresponds to amino acid residues 830-849 of human STAT2 protein.
STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon-alpha (IFN-alpha) and IFN-gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6).
STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5.
Specific functions of the various members of the STAT family are poorly understood. STAT1 and STAT2 form heterodimers that function as more potent inducers of transcription than the STAT1 homodimer. The unique acidic domain of the carboxy-terminal region of STAT2 may interact with cAMP-response-element binding protein. STAT2 has been shown to be activated by IFNalpha/beta and associate with the transcription factors p48, and P300.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: interferon alpha induced transcriptional activator; p113; p113 ; Signal transducer and activator of transcription 2; signal transducer and activator of transcription 2, 113kDa; Signal Transducers and Activators of Transcription 2
Gene Aliases: IMD44; ISGF-3; P113; STAT113; STAT2
UniProt ID: (Human) P52630
Entrez Gene ID: (Human) 6773
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